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目的 研究绞股蓝总皂苷对非酒精性脂肪肝(nonalcoholic fatty liver disease,NAFLD)大鼠肝脏保护作用及免疫调节作用机制.方法 48只SD大鼠随机分为正常对照组,模型对照组,多烯磷脂酰胆碱胶囊组(阳性对照组,150 mg·kg-1)及绞股蓝总皂苷高、中、低(240,120,60 mg·kg-1)剂量组,除正常对照组外,其余各组连续饲喂高脂饲料10周,制备NAFLD模型;模型成功后,各组连续给药8周.实验期间,分别于给药0,4,8周眼眶取血检测血清AST、ALT;末次给药后,采用流式细胞技术检测外周血Th17和Treg细胞含量;酶联免疫法检测血清IL-17、IL-10及TNF-α含量.HE染色检查肝组织病理变化和免疫组化法检查肝组织IL-17和Foxp3表达.结果 给药4周,绞股蓝总皂苷高剂量显著降低大鼠血清ALT、AST(P＜0.01);给药8周,绞股蓝总皂苷各剂量均能显著降低血清ALT、AST(P＜0.05,0.01).绞股蓝总皂苷高、中剂量能改善肝组织病变,降低TNF-α水平;高剂量显著降低IL-17、升高IL-10水平(P＜0.05,0.01),降低淋巴细胞IL-17含量,升高CD4+CD25+Treg含量(P＜0.05),明显减少炎症因子IL-17的表达和增加Foxp3表达.结论 绞股蓝总皂苷能改善NAFLD大鼠肝脏病变,其作用机制可能与调节肝脏Treg/Th 17细胞平衡,减少促炎症因子和增加抗炎因子产生相关.“,”OBJECTIVE To study the effect of stevenleaf on improving non-alcoholic fatty liver disease in rat model and clarify mechanism from the point of immune-regulation.METHODS Forty-eight of rats were divided into six groups:normal group,model group,Essentiale (150 mg·kg-1),high-dose (240 mg·kg-1),moderate-dose (120 mg·kg-1) and low-dose of stevenleaf (60 mg·kg-1) groups.Rats were given high fat food for continuous 10 weeks,then given correspond drugs for 8 weeks.The levels of serum AST and ALT were measured at 0,4 and 8 weeks experimental session.The rats were anesthetized after administration 8 weeks.The peripheral blood lymphocytes and serum were separated to measure the contents of Th17 and Treg cells in peripheral blood by flow cytometry and the levels ofIL-17,IL-10 and TNF-α in serum by enzyme-linked immunosorbent assay (ELISA).HE staining was used to observe the pathological changes of liver tissue and immunohistochemistry to observe the positive expression of IL-17 and Foxp3 in liver tissue.RESULTS The 240 mg·kg-1 of stevenleaf could reduce levels of serum ALT and AST after administration for 4 weeks (P＜0.01).stevenleaf 240 mg·kg-1,120 mg·kg-1 and 60 mg·kg-1 could reduce levels of serum ALT and AST after administration for 8 weeks (P＜0.05,0.01).Stevenleaf 240 mg·kg-1 and 120 mg·kg-1 could improve liver damage and reduce the level of TNF-α (P＜0.05).Stevenleaf 240 mg·kg-1 could reduce the content of IL-17 and increase IL-10 in serum (P＜0.05,0.01),reduce content of Th17 cell (CD4+IL-17+) and increase CD4+CD25+ Treg in peripheral blood lymphocytes (P＜0.05),also significantly reduced the expression of inflammatory factor IL-17 and increased Foxp3 expression.CONCLUSION Stevenleaf could protect liver tissue damages,its mechanism may be related to regulating Treg/Th1 7 cell balance,reducing pro-inflammatory factors and increasing anti-inflammatory factors.