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目的比较采暖期和非采暖期农村与城市居民体内DNA氧化损伤标志物8-羟基脱氧鸟苷(8-OHdG)的水平。方法分别于2009年10月(非采暖期)和2010年3月(采暖期),选择年龄在45～65周岁,在太原市居住时间≥5年的农村居民(傅山医院附近)79人和城市居民143人为研究对象,排除焦炉工等多环芳烃职业暴露者和癌症患者。采用ELISA试剂盒测定尿中8-OHdG的浓度。结果非采暖期农村居民尿中8-OHdG的浓度高于城市居民;采暖期农村居民尿中8-OHdG的浓度与城市居民无差别;在同一人群中,采暖期尿中8-OHdG的浓度高于非采暖期。结论太原市农村居民DNA氧化损伤水平高于城市居民,采暖期居民的DNA氧化损伤水平高于非采暖期。
Objective To compare the level of 8-hydroxydeoxyguanosine (8-OHdG), a DNA oxidative damage marker, in rural and urban areas during heating and non-heating periods. Methods A total of 79 rural residents (near Fu Shan Hospital) who lived in Taiyuan City for ≥ 5 years were selected from October 2009 (non-heating period) and March 2010 (heating period) 143 urban residents for the study, excluding coke oven workers and other occupational PAHs and cancer patients. The concentration of 8-OHdG in urine was determined by ELISA kit. Results The concentration of 8-OHdG in urine of rural residents during non-heating period was higher than that of urban residents. The concentration of 8-OHdG in urine was not different from that of urban residents in heating period. In the same population, the concentration of 8-OHdG in urine during heating period was high In non-heating period. Conclusion The level of DNA oxidative damage in rural residents in Taiyuan is higher than that in urban residents. The DNA oxidative damage of residents in Taiyuan was higher than that of non-urban residents during the heating period.