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应用杀雄菌属(Arsenophonus)23S rDNA基因和沃尔巴克氏体(Wolbachia)wsp基因的特异引物,通过PCR扩增的方法对我国20个水稻二化螟地理种群感染两类内共生菌的情况进行了检测.结果表明:我国水稻二化螟不同地理种群的Arsenophonus和Wolbachia感染率不一致,哈尔滨、惠水、吉林、南阳和扬州5个地理种群的Arsenophonus感染率为5.0%~50.0%;汉中、南宁和扬州3个地理种群的Wolbachia感染率为25.0%~40.0%,其他地理种群中没有检测到这两种共生菌的存在.水稻二化螟不同地理种群感染的Arsenophonus 23S rDNA基因序列完全一致,将该基因株型命名为csArs.但所检测到的Wolbachia wsp基因序列不一致,分别为wChisup1、wChisup5和wChisup6,其中wChisup1属于A群,其他属于B群.这说明水稻二化螟感染的Arsenophonus为同一株型,而感染的Wolbachia株型较为复杂.通过构建系统发育树发现,水稻二化螟体内的Arsenophonus 23S rDNA基因和Wolbachia wsp基因与其他物种体内相关序列完全一致或高度相似.
By using the primers of 23S rDNA gene of Arsenophonus and Wsp gene of Wolbachia, two types of endophytic bacteria were infected by the geographical population of 20 rice stem borers in China by PCR amplification The results showed that the infection rates of Arsenophonus and Wolbachia in different geographical populations of rice stem borer in China were inconsistent, and the infection rates of Arsenophonus in five geographic populations of Harbin, Huishui, Jilin, Nanyang and Yangzhou ranged from 5.0% to 50.0% The Wolbachia infection rates of three geographical populations in Nanning and Yangzhou ranged from 25.0% to 40.0%, and no other commensal bacteria were detected in other geographical populations.The sequence of Arsenophonus 23S rDNA was identical among different geographical populations of rice stem borer, The genotype was named as csArs, but the sequences of Wolbachia wsp gene were inconsistent, which were wChisup1, wChisup5 and wChisup6 respectively, of which wChisup1 belonged to group A and others belonged to group B. This indicated that Arsenophonus infected with S. Plant type, and infected Wolbachia plant type is more complicated by building phylogenetic tree found that the rice stem borer in vivo Arsenophonus 23S rDNA gene and W The olbachia wsp gene is completely or highly similar to other related species in vivo.