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采用农杆菌介导的子叶节遗传转化方法对16个大豆基因型进行转化,以丛生芽分化率和GUS阳性率作为指标,比较不同大豆基因型再生性及对农杆菌敏感性的差异。同时对大豆遗传转化再生过程中的种子萌发所需6-BA浓度、草丁膦(PPT)筛选压力等因素进行研究。结果表明:萌发培养基中添加适宜浓度的6-BA能显著提高丛生芽的分化率;不同大豆品种对PPT的敏感性不同,最适PPT浓度主要集中在5～7 mg.L-1;16个大豆基因型再生性及对农杆菌敏感性差异较大,其中合丰45和东农50的再生率及GUS表达率最高,是适合大豆遗传转化的基因型。
Sixteen soybean genotypes were transformed by agrobacterium-mediated cotyledonary node genetic transformation. Differences of regenerative and Agrobacterium sensitivities of different soybean genotypes were compared with the rate of clustered bud differentiation and GUS positive rate as indicators. At the same time, the factors of 6-BA concentration required for seed germination and screening pressure of glufosinate (PPT) during soybean genetic transformation and regeneration were studied. The results showed that the appropriate concentration of 6-BA in germination medium could significantly improve the differentiation rate of clustered buds. The sensitivity of different soybean varieties to PPT was different. The optimum concentration of PPT mainly concentrated in 5-7 mg.L-1; 16 The reproducibility of soybean genotypes and their sensitivity to Agrobacterium differed greatly. Among them, the regeneration rate and GUS expression rate of Hefeng 45 and Dongnong 50 were the highest, which were the most suitable genotypes for soybean genetic transformation.