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目的:建立中成药芪胶升白胶囊中朝藿定C和淫羊藿苷的含量测定方法。方法:采用高效液相色谱法,使用Diamonsil C18色谱柱(250 mm×4.6 mm,5μm),以乙腈-水(24.5∶75.5)为流动相,流速1 m L·min-1,检测波长270 nm,柱温25℃。结果:朝藿定C进样量在0.027~0.542μg范围内线性良好(r=0.999 8),淫羊藿苷进样量在0.025~0.499μg范围内线性良好(r=0.999 9)。朝藿定C的平均回收率(n=6)为99.27%,RSD为1.2%;淫羊藿苷的平均回收率(n=6)为100.2%,RSD为1.6%。测得3批次芪胶升白胶囊中的朝藿定C、淫羊藿苷的平均含量范围为0.570~0.599 mg·g-1、0.395~0.409 mg·g-1。结论:本方法经方法学验证,可作为芪胶升白胶囊中朝藿定C和淫羊藿苷含量测定的方法。
OBJECTIVE: To establish a method for the determination of epimedin C and icariin in Chinese traditional medicine Shengli Capsule. METHODS: Diamonsil C18 column (250 mm × 4.6 mm, 5 μm) with acetonitrile-water (24.5: 75.5) was used as the mobile phase at a flow rate of 1 mL · min-1 and the detection wavelength was set at 270 nm , Column temperature 25 ℃. RESULTS: The injection volume of Epimedin C was linear in the range of 0.027-0.542 μg (r = 0.999 8). The linearity of icariin injection in the range of 0.025-0.499 μg was good (r = 0.999 9). The average recovery of epimedin C (n = 6) was 99.27% and the RSD was 1.2%. The average recovery of icariin (n = 6) was 100.2% and the RSD was 1.6%. Measured epimedin C in three batches of QGLB, the average contents of icariin ranged from 0.570 to 0.599 mg · g -1 and from 0.3395 to 0.409 mg · g -1. Conclusion: This method is validated by methodology and can be used as a method for determination of Epimedin C and icariin in QGLB.