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目的比较室内繁殖东方田鼠与昆明鼠感染日本血吸虫后不同阶段脏器病变以及体内日本血吸虫发育与存活状况。方法采用日本血吸虫尾蚴感染健康东方田鼠和昆明鼠,在感染后12、20、40 d剖杀,观察并比较东方田鼠以及昆明鼠肝、肾、肺等脏器组织病变及体内日本血吸虫发育与存活情况。结果日本血吸虫感染后12、20 d昆明小鼠和感染40d后东方田鼠的内脏器官均未出现明显病变;感染12 d及20 d后的东方田鼠肝脏、肾脏及脾脏出现明显白色结节,且以感染12 d的组织病变更为明显,部分东方田鼠病变组织仅见于肝脏。病理切片显示,病变肝脏和肾脏组织中存在完整日本血吸虫虫体,与正常组织界限分明;而无病变的肝脏和肾脏组织病理切片未发现虫体。结论室内繁殖东方田鼠感染日本血吸虫后12 d免疫反应剧烈且存在明显的个体差异。
Objective To compare the different stages of organ disease and the development and survival of Schistosoma japonicum in vivo after inoculation of Microtus fortis and Kunming mice with Schistosoma japonicum. Methods Healthy cistocathenus voles and Kunming mice were infected with cercariae of Schistosoma japonicum, and were dissected at 12, 20 and 40 days after infection. The histopathological changes of liver and kidney, lungs and other tissues of Microtus fortis and Kunming mice were observed and compared. The development and survival of Schistosoma japonicum Happening. Results No obvious pathological changes were observed in the organs of Kunming mice and Microtus fortis at 12 and 20 days after infection with Schistosoma japonicum. Significant white nodules appeared in the liver, kidney and spleen of Microtus fortis at 12 d and 20 d after infection Tissue lesions were more evident on 12 d of infection, and some of the lesion voles were only found in the liver. Pathological sections showed that there was a complete schistosoma japonicum body in diseased liver and kidney tissues, which was distinct from normal tissues. No lesions were found in diseased liver and kidney tissues. CONCLUSION: The immune response of T. mandarinus infected with Schistosoma japonicum 12 days after inbreeding was violent and there were obvious individual differences.