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利用自国外引进的双拷贝乙型肝炎病毒DNA重组质粒进行改造。用内切酶部分消化收取分子量大小不同的含有乙型肝炎表面抗原(HBsAg)的基因片段,插入另一含有二氢叶酸还原酶(dhfr)基因的质粒中进行重组,重新构造成功4种插入片段大小不同及转录方向不
Using imported from abroad double copy hepatitis B virus DNA recombinant plasmid transformation. The gene fragment containing hepatitis B surface antigen (HBsAg) with different molecular weight was partially digested with endonuclease and inserted into another plasmid containing dihydrofolate reductase (dhfr) gene for recombination. Four kinds of inserted fragments Different size and transcription direction is not