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目的:优选板蓝根软化切制的工艺条件;通过比较不同产地板蓝根药材切制前后指纹图谱的差异性,客观反应润药切制对药材总体成分的影响。方法:以药材软化时加水量、润制时间和饮片切制厚度为考察因素,以水溶性浸出物为指标,采用正交设计,优选板蓝根软化切制的条件;采用反相高效液相色谱法,Zorbax SB-C18(4.6 mm×250 mm,5μm)柱,流动相乙腈-水,梯度洗脱,检测波长238 nm,进样量10μL。对板蓝根粗粉和水润切制后的饮片进行HPLC测定,并采用中药色谱指纹图谱相似度评价软件进行比较。结果:板蓝根软化切制的最佳工艺为:0.6倍量的水,浸润24 h,饮片厚度为2 mm;结论:建立了板蓝根药材醋酸乙酯萃取物HPLC指纹图谱。板蓝根在切制前后,指纹图谱对照谱图差异明显,切制后中等极性部分损失较多。
Objective: To optimize the process conditions for the softening of Radix Isatidis. By comparing the difference of fingerprint before and after cutting Radix Isatidis from different areas, objectively reflect the influence of the elixir cutting on the total composition of the medicinal materials. Methods: According to the amount of water when softening, the time of curing and the cut thickness of slices, the orthogonal design was adopted to optimize the cut conditions of Radix isatidis with water-soluble extract as the index. The optimum conditions were determined by reversed-phase high performance liquid chromatography (4.6 mm × 250 mm, 5 μm). The mobile phase consisted of acetonitrile-water and gradient elution. The detection wavelength was 238 nm and the injection volume was 10 μL. The content of Radix isatidis and Hydrolyzed Herbal Pieces were measured by HPLC and compared with the similarity evaluation software of chromatographic fingerprints of traditional Chinese medicine. Results: The optimum conditions for the softening of Radix isatidis were as follows: 0.6 times the amount of water, infiltration for 24 h, the thickness of the slices was 2 mm; Conclusion: The HPLC fingerprint of ethyl acetate extract of Radix isatidis was established. Radix before and after cutting, fingerprinting control spectra significantly different, after cutting the middle part of the more polar loss.