论文部分内容阅读
目的观察血管内皮细胞生长因子(vascularendothelialgrowthfactor, VEGF)能否改变血迷路屏障和血外淋巴屏障的物质转运作用。方法11只300 ~900g雌雄兼并的杂色豚鼠在甲苯噻嗪(16mg/kg)和氯氨酮(60mg/kg)基础麻醉下接受手术,通过圆窗膜(明胶海绵吸附)将VEGF(6耳)或磷酸盐缓冲液(PBS, 5耳)投放至内耳。用T1对比剂二乙烯三胺五乙酸双甲酰胺钆(gadolinium diethylenetriamine pentaacetate bismethylamide, Gd DTPA BMA)作为内耳屏障转运示踪剂,用4 7T场强, 40cm孔径的BrukerBiospecAvance47 /40试验磁共振系统进行豚鼠耳蜗二维磁共振成像观测,用Paravision软件进行图像密度分析,用AdobePhotoshop6 0软件进行图像呈示。结果圆窗膜投放PBS未影响血外淋巴屏障渗透性变化。圆窗膜投放VEGF可显著增强处理侧血外淋巴屏障渗透性,VEGF处理的耳蜗鼓阶内Gd DTPA BMA转运显著增加(P<0 01 ), VEGF处理的耳蜗前庭阶内Gd DTPA BMA转运也显著增加(P<0 01)。VEGF处理的耳蜗中阶内Gd DTPA BMA转运无明显增加(P>0 05)。结论VEGF显著增强血外淋巴屏障的物质转运作用并可能有利于内耳在各种有害环境下的代偿及修复。
Objective To observe whether vascularendothelial growth factor (VEGF) can change the substance transport of blood labyrinth barrier and extralymphatic lymph barrier. Method 11 Mature male and female pigmented guinea pigs undergoing surgical operation under basic anesthesia with xylazine (16 mg / kg) and ketamine (60 mg / kg) were anesthetized with 300 to 900 g of VEGF (6 ears ) Or phosphate buffered saline (PBS, 5 ears) to the inner ear. A T1 contrast agent, gadolinium diethylenetriamine pentaacetate bismethylamide (Gd DTPA BMA), was used as a tracer for inner ear barrier transport. Guinea pigs were treated with a 4 7T field strength Bruker Biospec Avance 47/40 magnetic resonance system with a 40 cm aperture Cochlear two-dimensional magnetic resonance imaging observation, using Paravision software for image density analysis, with AdobePhotoshop60 software for image rendering. Results The round window membrane administration of PBS did not affect the permeability change of the extralymphatic lymph barrier. The round window membrane administration of VEGF significantly enhanced the permeability of the peripheral blood-brain barrier on the treatment side, and the transport of Gd DTPA BMA was significantly increased (P <0.01) in the VEGF-treated cochlea and the VEGF-treated vestibular Gd DTPA BMA transport was also significant Increase (P <0.01). There was no significant increase in intracellular Gd DTPA BMA translocation in VEGF-treated cochleas (P> 0.05). Conclusions VEGF significantly enhances substance transport in the extralymphatic lymphatic barrier and may be beneficial to the compensation and repair of the inner ear in various harmful environments.