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目的建立Lewis肺癌移植瘤模型,人血管能抑素(canstatin)基因重组表达载体电穿孔法转染至肿瘤局部,观察对肿瘤生长和转移的抑制作用。方法将Lewis肺癌细胞接种于C57BL小鼠皮下,成瘤后将30只小鼠随机分成3组,分别瘤体内注射血管能抑素重组载体、空载体和生理盐水,基因枪于注射部位电击,2周后PT-PCR法检测肿瘤组织中血管能抑素基因的表达并观察小鼠体重、肿瘤体积变化及肺转移结节数目。结果成功建立Lewis肺癌皮下移植瘤模型;电击2周后重组载体组仍存在血管能抑素基因的表达,重组载体组小鼠肿瘤体积由(1.03±0.11)cm3增加至(1.51±0.19)cm3,而空载体组由(1.01±0.09)cm3增至(2.45±0.18)cm3、NS组由(1.02±0.10)cm3增至和(2.54±0.21)cm3;重组载体组体积与后两组比较(P<0.01),即生长速度受到显著的抑制。重组载体组、空载体组和NS组的肺转移结节数分别为(3.50±1.24),(7.60±2.51)和(7.70±2.45);重组载体组与后两组比较(P<0.01),即肿瘤转移亦受到显著的抑制。结论血管能抑素可明显抑制Lewis肺癌移植瘤的生长与转移。
OBJECTIVE: To establish Lewis lung carcinoma xenograft model and transfect the recombinant human vascular canstatin gene into the tumor site by electroporation. The inhibitory effect on tumor growth and metastasis was observed. Methods Lewis lung carcinoma cells were inoculated subcutaneously into C57BL mice. Thirty mice were randomly divided into three groups after tumorigenesis. The mice were injected with either angiotensin-containing recombinant vector, empty vector and saline, The expression of endostatin gene in tumor tissue was detected by PT-PCR and the body weight, tumor volume and number of lung metastasis were observed. Results The subcutaneous xenograft model of Lewis lung carcinoma was successfully established. The expression of angiostatin gene was still observed in the recombinant vector after 2 weeks of shock. The tumor volume of the recombinant vector group increased from (1.03 ± 0.11) cm3 to (1.51 ± 0.19) cm3, (1.01 ± 0.09) cm3 to (2.45 ± 0.18) cm3 in NS group and from (1.02 ± 0.10) cm3 to (2.54 ± 0.21) cm3 in NS group. The volume of recombinant vector group was significantly higher than that in the latter two groups <0.01), that is, the growth rate was significantly inhibited. The numbers of pulmonary metastasis in the recombinant vector group, empty vector group and NS group were (3.50 ± 1.24), (7.60 ± 2.51) and (7.70 ± 2.45), respectively. The recombinant vector group and the latter two groups were significantly (P <0.01) That tumor metastasis was also significantly inhibited. Conclusion Angiostatin can significantly inhibit the growth and metastasis of Lewis lung carcinoma.