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为提高决明幼苗在盐胁迫条件下的抗逆能力,该研究采用外施Ca2+,SA及NO对盐胁迫下决明幼苗的光合及生理特性进行了研究.结果表明:盐胁迫条件下决明幼苗生理受到严重影响,3种外源物质均可在一定程度上缓解盐胁迫对决明幼苗造成的伤害.其中在外施20mmol/L的Ca2+,0.25mmol/L的NO及100mg/L的SA处理下,幼苗叶片各项指标均与盐胁迫组差异显著,接近空白对照.3种外源物质中,0.25mmol/L的外源NO处理可以较好地缓解盐胁迫对决明幼苗的伤害.在此处理下,幼苗叶片可溶性蛋白、总叶绿素质量分数分别达25.68mg/g,3.52 mg/g;MDA,O2-.降低至774.03μg/g,33.60μg/g;超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)和抗坏血酸过氧化物酶(APX)活性均显著升高,分别达到了65.12 U/g.min,152.61U/g.min,161.33U/g.min和104.12U/g.min;其净光合速率达到了6.95μmol/m2.s,超过了空白对照组(6.92μmol/m2.s).
In order to improve the resistance of Cassia obtusifolia seedlings under salt stress, the photosynthesis and physiological characteristics of Cassiai seedlings under salt stress were studied by applying Ca2 +, SA and NO.Results showed that under salt stress, Seedling physiology was seriously affected, and all the three exogenous substances could alleviate the damage caused by salt stress to Cassia seedlings to a certain extent.Under 20 mmol / L Ca2 +, 0.25mmol / L NO and 100mg / L SA treatment , The indexes of seedling leaves were significantly different from those of salt stress group, close to the blank control.Of three kinds of exogenous substances, 0.25mmol / L exogenous NO could alleviate the damage of salt stress to Cassiai seedlings, , The content of soluble protein and total chlorophyll in seedling leaves were 25.68mg / g and 3.52 mg / g respectively; the content of MDA and O2- decreased to 774.03μg / g and 33.60μg / g; the content of superoxide dismutase The activities of CAT, POD and APX were significantly increased, reaching 65.12 U / g.min, 152.61U / g.min, 161.33U / g .min and 104.12U / g.min, respectively. The net photosynthetic rate reached 6.95μmol / m2.s, which was higher than that of the blank control group (6.92μmol / m2.s).