论文部分内容阅读
研究了5种稀土元素(镧、铈、钕、镨、铒)对桑黄液体发酵的影响。实验结果表明,不同的稀土元素在适宜浓度时对桑黄生物量以及多糖、三萜和黄酮等活性物质的合成,均具有一定的促进作用。其中稀土元素铈和铒有利于桑黄生物量的积累,并分别在浓度0.01 mmol.L-1和0.1 mmol.L-1时使生物量达到最大值(5.85±0.14)g.L-1和(5.34±0.45)g.L-1,比对照提高了23%和13%。而稀土元素铈、钕和镧则对桑黄胞外多糖、胞内多糖和胞内三萜的合成具有明显促进作用,其中铈在浓度0.01 mmol.L-1、钕在浓度0.1 mmol.L-1、铈在0.01 mmol.L-1时分别使胞外多糖、胞内多糖和胞内三萜产量达到最大值(610.36±0.31)mg.L-1、(789.09±2.09)mg.L-1和(106.70±2.92)mg.L-1。本文所考察的5种稀土元素都在一定程度上促进了桑黄胞外三萜和黄酮的合成,但稀土元素镧、钕和镨对胞外三萜诱导效果相对较好,而钕、铒和铈则对黄酮诱导效果相对较好。综合来看,稀土元素对桑黄液体发酵的影响,与稀土元素的种类和浓度密切相关,体现出一定的选择性和特异性。
The effects of five rare earth elements (lanthanum, cerium, neodymium, praseodymium and erbium) on the fermentation of Phellinus igniarius were studied. The experimental results showed that different rare earth elements had certain promotion effect on the biosynthesis of polysaccharide, triterpene and flavonoids at the proper concentration. Among them, rare earth elements cerium and erbium were beneficial to the accumulation of Phellinus igniarius biomass and reached the maximum (5.85 ± 0.14) gL-1 and (5.34) at the concentrations of 0.01 mmol.L-1 and 0.1 mmol.L-1, respectively ± 0.45) gL-1, up 23% and 13% over the control. While rare earth elements such as cerium, neodymium and lanthanum significantly promoted the synthesis of polysaccharide, intracellular polysaccharide and intracellular triterpene, with the concentrations of cerium at 0.01 mmol.L-1 and neodymium at 0.1 mmol.L- 1, the maximum production of exopolysaccharides, intracellular polysaccharides and intracellular triterpenes were reached at the concentrations of 0.01 mmol.L-1 (610.36 ± 0.31) mg.L-1 and (789.09 ± 2.09) mg.L-1, respectively And (106.70 ± 2.92) mg.L-1. The five rare earth elements investigated in this paper all promoted the synthesis of extracellular triterpenoids and flavonoids in P. epidermidis to some extent. However, the effects of rare earth elements such as lanthanum, neodymium and praseodymium on the induction of extracellular triterpenes were relatively good, while neodymium, erbium and Cerium is relatively good for the induction of flavonoids. Taken together, the effect of rare earth elements on the fermentation of Phellinus igniarius is closely related to the species and concentration of rare earth elements, which shows some selectivity and specificity.