转基因抗病毒病四倍体西瓜的培育

来源 :中国瓜菜 | 被引量 : 0次 | 上传用户：benben1906

【摘要】

：

利用西瓜花叶病毒2号(WMV-2)外壳蛋白(CP)基因、小西葫芦黄花叶病毒(ZYMV)复制酶(NIb)基因和黄瓜花叶病毒(CMV)复制酶基因构建三价基因的植物表达载体,通过农杆菌介导转化四

【作者】

：

黄学森牛胜鸟王锡民于嘉林赵福兴王生有翟光明蔡晓

【机构】

：

山西金鼎生物种业股份有限公司,中国农业大学生物技术,新加坡国立大学

【出处】

：

中国瓜菜

【发表日期】

：

2004年期

【关键词】

：

四倍体西瓜病毒病转基因抗病性

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论文部分内容阅读

利用西瓜花叶病毒2号(WMV-2)外壳蛋白(CP)基因、小西葫芦黄花叶病毒(ZYMV)复制酶(NIb)基因和黄瓜花叶病毒(CMV)复制酶基因构建三价基因的植物表达载体,通过农杆菌介导转化四倍体西瓜植株,经分子检测证明目的基因成功地导入西瓜植株,并在后代中稳定遗传.经温室及大田接种鉴定,T3代转基因西瓜抗病毒性达中抗水平,为抗病毒无籽西瓜新品种的培育提供了可能性.

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转基因抗病毒病四倍体西瓜的培育

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