论文部分内容阅读
目的:探讨慢传输型便秘患者结肠组织中肥大细胞浸润及microRNA-128表达的关系及其临床意义。方法:通过免疫组织化学技术检测慢传输型便秘患者结肠组织肥大细胞浸润,通过实时荧光定量聚合酶链反应(RT-qPCR)检测慢传输型便秘患者结肠组织microRNA-128表达情况,并分析两者表达的相关性。结果:与正常结肠组织比较,慢传输便秘患者结肠组织内肥大细胞浸润显著增加(P<0.05),microRNA-128表达水平显著降低(P<0.05),且两者呈显著负相关(P<0.01)。结论:结肠组织内肥大细胞浸润增加及microRNA-128表达降低可能参与了慢传输型便秘的发病机制。
Objective: To investigate the relationship between mast cell infiltration and the expression of microRNA-128 in colonic tissue of patients with chronic transit constipation and its clinical significance. Methods: Mast cell infiltration was detected by immunohistochemistry in colorectal mucosa of patients with slow transit constipation. The expression of microRNA-128 in colon tissues of patients with chronic transit constipation was detected by real-time fluorescence quantitative polymerase chain reaction (RT-qPCR) Correlation of expression. Results: Compared with normal colon tissues, the infiltration of mast cells in colon tissues of slow-transit constipation patients was significantly increased (P <0.05) and the expression of microRNA-128 was significantly decreased (P <0.05) ). CONCLUSIONS: Increased mast cell infiltration and reduced expression of microRNA-128 in colonic tissues may be involved in the pathogenesis of slow transit constipation.