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为了解蓝藻暴发池塘中细菌群落特征,采集3个地区(广东、云南、贵州)4个淡水养殖场的蓝藻暴发池塘和非蓝藻暴发池塘(对照池塘)水样,并检测其理化因子及生物指标,采用PCR-DGGE技术分析其细菌群落结构差异。依据PCR-DGGE指纹谱带的丰度对养殖水体细菌群落多样性进行了分析,并对水体细菌群落结构进行了UPGMA聚类分析。结果发现:蓝藻暴发池塘水体的PO_4-P含量均显著高于对照池塘(P<0.01);线性回归分析表明,PO_4-P与代表蓝藻暴发程度的叶绿素a存在正相关关系(R~2=0.869,P<0.01);而且理化因子与细菌群落的RDA分析表明,PO_4-P与蓝藻暴发池塘细菌群落关系密切。蓝藻暴发池塘与对照池塘水体的细菌群落结构间存在显著差异;进一步测序分析显示,蓝藻暴发池塘特定的细菌为Flexibacter,其可能对蓝藻有裂解作用;而Synechococcsus在蓝藻暴发池塘的含量明显低于对照池塘,可能是Microcystis的大量暴发抑制了Synechococcsus的生长。
In order to understand the characteristics of bacterial community in cyanobacteria outbreak ponds, the water samples of cyanobacteria outbreak ponds and non-cyanobacteria outbreak ponds (control ponds) in 4 freshwater aquaria farms in 3 regions (Guangdong, Yunnan and Guizhou) were collected and their physico-chemical factors and biomarkers , PCR-DGGE analysis of bacterial community structure differences. Based on the abundance of PCR-DGGE fingerprinting bands, the diversity of bacterial community in aquaculture water was analyzed, and the UPGMA cluster analysis of bacterial community structure was carried out. The results showed that the content of PO 4 -P in the pond water of cyanobacteria outbreak was significantly higher than that in the control pond (P <0.01). The linear regression analysis showed that there was a positive correlation between PO 4 -P and chlorophyll a which represented the degree of cyanobacteria outbreak (R 2 = 0.869 , P <0.01). Moreover, RDA analysis of physico-chemical factors and bacterial community showed that PO 4 -P was closely related to the bacterial community of cyanobacteria outbreak pond. The results showed that there was a significant difference in bacterial community structure between cyanobacteria outbreak pond and control pond water body. Further sequencing analysis showed that the specific bacterium in the cyanobacteria outbreak pond was Flexibacter, which may have a cracking effect on cyanobacteria; however, the content of Synechococcsus in the outbreak pond of cyanobacteria was significantly lower than that of the control Ponds, probably a large outbreak of Microcystis, inhibited the growth of Synechococcsus.