AIM:To study changes of inflammation-associated cytokineexpressions during early phase of endotoxic shock in macague.METHODS:Experiments were performed in Macaquemulatta treated with LPS 2.8 mg/kg in shock model groupor with normal saline in control group.Blood samples werecollected before,or 60 rain,or 120 min after LPS injection,respectively.Liver and spleen tissues were obtained at120 min after LPS injection.The plasma levels of TNF-α,IL-1 β,IL-10 and IL-12P40 were determined by double-antibody sandwich ELISA with antibodies against humancytokines.The mRNA levels of TNF-α,IL-1 β,and IL-18 inperipheral blood mononudear cells (PBMCs),liver and spleenwere examined by real-time fluorescence semi-quantitativeRT-PCR with the primers based on human genes.RESULTS:Mean systemic arterial pressure(MAP),systemicvascular resistance index(SVRI)and left ventricular workindex(LVWI)of macaques were significant declined in shockmodel group on average 60 min after LPS injection.Theplasma levels of TNF-α and IL-10 were significantlyincreased 60 rain after LPS injection and then decreased.The plasma levels of IL-1 β and IL-12P40 were significantlyincreased at 120 min after LPS injection.The mRNA levelsof TNF-α and IL-1 β were significantly increased 60 minafter LPS stimulation in PBMCs and 120 rain after LPSstimulation in livers.The mRNA level of IL-18 wassignificantly increased 120 min after LPS stimulation inPBMCs and livers.But in spleen,only TNF-α mRNA level inLPS group was significantly higher 120 rain after LPSstimulation,compared with that in control group.CONCLUSION:An endotoxic shock model of Macaquemulatta was successfully established.Both antibodies forELISA and PCR primers based on human cytokine assayswere successfully applied to detect macaque cytokines.Inthe model,inflammatory cytokines,such as TNF-α,IL-1 β,IL-12 and IL-18 as well as anti-inflammation cytokine IL-10,were released at very early phase of endotoxic shock within120 min after LPS injection.PBMCs and liver cells might bethe important sources of these cytokines. AIM: To study changes of inflammation-associated cytokineexpressions during early phase of endotoxic shock in macague. METHODS: Experiments were performed in Macaquemulatta treated with LPS 2.8 mg / kg in shock model groupor with normal saline in control group. Blood samples were collected before, or 60 min, or 120 min after LPS injection, respectively. Liver and spleen tissues were obtained at 120 min after LPS injection. Plasma levels of TNF-α, IL-1 β, IL-10 and IL-12P40 were determined by double-antibody sandwich ELISA with antibodies against human cytokines. The mRNA levels of TNF-α, IL-1β, and IL-18 in peripheral blood mononudear cells (PBMCs), liver and spleenwere examined by real-time fluorescence semi-quantitative RT- on human genes.RESULTS: Mean systemic arterial pressure (MAP), systemicvascular resistance index (SVRI) and left ventricular workindex (LVWI) of macaques were significantly less in shockmodel group on average 60 min after LPS injection. TNF-α and IL-10 were significantly increased by 60 min after LPS injection and then decreased. Plasma levels of IL-1 β and IL-12P40 were significantly increased at 120 min after LPS injection. MRNA levels of TNF-α and IL-1β were significantly increased by 60 m LPS stimulation in PBMCs and 120 min after LPS stimulation in livers. mRNA levels of IL-18 wassignificantly increased by 120 min after LPS stimulation in PBMCs and livers.But in spleen, only TNF-α mRNA level in LPS group was significantly higher 120 rain after LPS stimulation, compared with that in control group. CONCLUSION: An endotoxic shock model of Macaquemulatta was successfully established. The Thrombocytes for ELISA and PCR primers based on human cytokine assays successfully to detect macaque cytokines. Inthe model, inflammatory cytokines, such as TNF-α, IL-1β, IL-12 and IL-18 as well as anti-inflammation cytokine IL-10, were released at very early phase of endotoxic shock within 120 min after LPS injection. PBMCs and livercells might be the important sources of these cytokines.