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建立了利用高效液相色谱法同时测定玉米籽粒类胡萝卜素和生育酚各组分含量的技术体系,分析了112份黄色胚乳玉米自交系的类胡萝卜素和生育酚含量,其中包括32份高油自交系和80份在生产上广泛应用的普通玉米自交系。结果表明,不同自交系间存在广泛的变异,各组分含量变幅最大的是α-生育酚,含量相差达162倍;变幅最小的是δ-生育酚,含量相差也接近4倍。类胡萝卜素各组分含量在高油和普通玉米自交系之间并没有显著差异,但普通玉米的变异范围更为广泛;高油玉米生育酚各组分含量显著高于普通玉米,其中γ-生育酚、α-生育酚和总生育酚含量的均值分别是普通玉米均值的2.4、2.5和2.4倍。尽管供试的高油玉米材料仅32份,但其生育酚的某些组分比普通玉米具有更广泛的遗传变异。这为进一步的玉米高油、高维生素A原、高维生素E,即“三高”品质成分育种提供了有益参考。
A technical system for the simultaneous determination of carotenoids and tocopherols in corn by HPLC was established. The contents of carotenoid and tocopherol in 112 yellow endosperm maize inbred lines were analyzed, including 32 high Oil inbred lines and 80 common maize inbred lines widely used in production. The results showed that there were a wide range of variation among the different inbred lines. The content of α-tocopherol was the largest, with a content difference of 162 times; the smallest amplitude was δ-tocopherol, the content difference was close to 4 times. Carotenoid content of high oil and common maize inbred lines between the no significant difference, but common maize mutated more widely; high oil corn tocopherol content was significantly higher than ordinary corn, where γ - The mean levels of tocopherols, alpha-tocopherol and total tocopherols were 2.4, 2.5 and 2.4 times the average for normal maize, respectively. Although only 32 samples of high-oil corn were tested, some components of their tocopherols had a broader genetic variation than common corn. This provides a useful reference for further breeding of corn high oil, high vitamin A, high vitamin E, that is, “three high” quality ingredients.