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高效液相色谱(HPLC)分析中,溶剂脱气是非常重要的步骤。因为在高的进柱压力下,溶解在移动相中的空气会在柱出口压力下降时形成气泡,从而严重干扰多种检测器(特别是光学检测器),使色谱图中出现严重的基线波动和气泡峰而妨碍组分峰的检出。通常使用减压、超声波振动或通入在液体中溶解度比空气小的惰性气体进行溶剂脱气。岛津LC—4A型仪器内附有专门装置,在溶剂进入泵系统之前通入氦气脱气。由于氦气难以购买,曾
In high performance liquid chromatography (HPLC) analysis, solvent degassing is a very important step. Because at high column pressures, air dissolved in the mobile phase creates bubbles as the column outlet pressure drops, severely disrupting multiple detectors (especially optical detectors) and causing severe baseline fluctuations in the chromatogram And bubble peak hinder the detection of component peaks. Solvent degassing is usually carried out using reduced pressure, ultrasonic vibrations, or inert gas, which is less soluble in the liquid than air. Shimadzu LC-4A-type instrument equipped with a special device, the solvent into the pump system before the introduction of helium degassing. Since helium is hard to buy, ever