根据莠去津均三氮苯类结构的特征，合成连有活性羧基的莠去津半抗原，通过活性脂法与牛血清白蛋白联接，制备出较高生物活性的人工抗原，以此免疫兔子获得抗莠去津的多克隆抗体。抗体的特异性强，与扑灭津的交叉反应性为９３％，与西玛津的交叉反应性为８％。以３Ｈ标记莠去津建立的莠去津放射免疫测定法对土壤中莠去津添加回收率的测定表明，沙土的添加回收率为８５７％～９５０％，壤土的添加回收率为８６９％～９２１％。 According to the characteristics of the atrazine triazine structure, the synthetic atrazine-containing hapten with active carboxyl group was synthesized and linked to bovine serum albumin by the active lipid method to prepare the artificial antigen with higher biological activity, so as to immunize the rabbit Anti-atrazine anti-polyclonal antibodies were obtained. Antibody specificity was strong, cross-reactivity with fenvastatin was 93%, and cross-reactivity with simazine was 8%. The determination of atrazine reabsorption rate in soil by atrazine radioimmunoassay with 3H-labeled atrazine showed that the recovery rate of added atrazine was 857% -950%, and that of loamy soil was 86  9% ~ 92  1%.