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目的：探讨干扰素（IFN）对肝癌细胞HLA－DR表达的调节作用。方法：采用免疫组化ABC法和ELISA法分别检测了不同剂量的IFN－γ或IFN－α刺激前后HLA－DR在HHCC、SMMC－7721、BEL－7402和HCC－9204等4种人肝细胞癌细胞系以及正常人肝细胞系QZG的表达情况。结果：IFN－γ或IFN－α刺激之前，5种细胞系中只有少数的SMMC－7721细胞可表达HLA－DR；刺激之后，对HLA－DR的表达5种细胞系都有所增强，并且与IFN－γ的剂量呈正相关，QZG不如肝癌细胞明显，IFN－γ的作用强于IFN－α。结论：IFN可以上调肝癌细胞对HLA－DR的表达。关键词：##4干扰素;;HLA－DR;;肝细胞癌;;免疫组织化学;;ELISA
Objective: To investigate the effect of interferon (IFN) on the regulation of HLA-DR expression in hepatoma cells. METHODS: Immunohistochemical ABC method and ELISA method were used to detect four human hepatocellular carcinomas of HLA-DR before and after stimulation with different doses of IFN-γ or IFN-α in HHCC, SMMC-7721, BEL-7402 and HCC-9204, respectively. Expression of QZG in cell lines and normal human hepatic cell lines. Results: Before stimulation with IFN-γ or IFN-α, only a small number of SMMC-7721 cells in 5 cell lines could express HLA-DR; after stimulation, the 5 cell lines of HLA-DR expression were enhanced, and The dose of IFN-γ was positively correlated, QZG was not as obvious as hepatoma cells, and IFN-γ was stronger than IFN-α. Conclusion: IFN can upregulate the expression of HLA-DR in liver cancer cells. Key words: ##4 interferon; HLA-DR; hepatocellular carcinoma; immunohistochemistry; ELISA