十全大补汤对乳腺癌患者术后早期恢复及切口引流液血管生成因子的影响

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目的:观察十全大补汤对乳腺癌患者术后早期恢复的作用,并探讨对切口引流液血管生成因子的影响以及引流液对乳腺癌细胞MDA-MB-231增殖、迁移和侵袭能力的作用。方法:将61例乳腺癌病例随机分为对照组30例和治疗组31例,两组术后均采用西医常规治疗,治疗组于术后第1天起服用十全大补汤,连续7 d;观察两组患者治疗前后中医证候疗效、切口引流时间及不同时点引流量,CCK-8法检测引流液对MDA-MB-231细胞增殖的影响、Transwell法检测细胞迁移、侵袭能力的变化,蛋白质芯片法筛选两组引流液中血管生成因子的表达。结果:(1)十全大补汤组中医证候疗效总有效率为89.66%,高于对照组的63.33%(P<0.05)。(2)十全大补汤组切口引流时间短于对照组引流时间,分别为(12.48±2.11)d、(14.23±1.94)d(P<0.05)。两组引流量均呈下降趋势,其中第7天十全大补汤组引流量低于对照组(P<0.05)。(3)引流液对MDA-MB-231细胞有促增殖作用,十全大补汤组引流液对MDA-MB-231乳腺癌细胞的促增殖作用低于对照组(P<0.05)。(4)Transwell结果发现引流液能促进MDA-MB-231细胞迁移和侵袭能力,而十全大补汤组的引流液促迁移和侵袭能力较对照组降低(P<0.05)。(5)人血管生成因子抗体芯片筛选发现43种血管生成因子在引流液中均有表达,部分促血管生成因子上调≥2倍,其中十全大补汤组IL-8、Leptin的表达水平低于对照组(P<0.05)。结论:十全大补汤能改善乳腺癌患者术后早期气血两虚症候,有效缩短术区引流时间、减少术区引流量;切口引流液能促进MDA-MB-231的增殖、迁移及侵袭,十全大补汤可能通过降低引流液中IL-8、Le Ptin等促血管生成因子的表达进一步抑制引流液对MDA-MB-231细胞的促增殖、迁移及侵袭能力。 OBJECTIVE: To observe the effect of Shiquan Dabu Tang on early postoperative recovery of breast cancer patients, and to explore the effect on the angiogenesis factor of incision drainage fluid and the effect of drainage fluid on the proliferation, migration and invasion of breast cancer cell MDA-MB-231 . Methods: Sixty-one cases of breast cancer were randomly divided into control group (n = 30) and treatment group (n = 31). Both groups were treated routinely with western medicine. After treatment, Shiquan Dabu Tang was taken on the first day after operation for 7 days The curative effect of traditional Chinese medicine (TCM), the time of incision drainage and drainage at different time points were observed before and after treatment. The effects of drainage fluid on the proliferation of MDA-MB-231 cells were detected by CCK-8 assay. The changes of cell migration and invasion were detected by Transwell assay Protein chip was used to screen the expression of angiogenic factors in the two drainage fluids. Results: (1) The total effective rate of TCM syndromes in Shiquan Dabu Tang is 89.66%, which is higher than that in control group (63.33%, P <0.05). (2) The duration of incision drainage in Shiquanbabu Decoction group was shorter than that in control group (12.48 ± 2.11 days, 14.23 ± 1.94 days, respectively) (P <0.05). Drainage volume of both groups showed a downward trend, of which Shi Dafu Tang group drainage on the seventh day was lower than the control group (P <0.05). (3) Drainage fluid could promote the proliferation of MDA-MB-231 cells. The effect of Shuntutang decoction drainage fluid on the proliferation of MDA-MB-231 breast cancer cells was lower than that of the control group (P <0.05). (4) Transwell results showed that the drainage fluid can promote the migration and invasion ability of MDA-MB-231 cells, while the drainage and invasion ability of Shiquandabutang group decreased compared with the control group (P <0.05). (5) Human angiogenic factor antibody chip screening found that 43 kinds of angiogenesis factors were expressed in the drainage fluid, some of the pro-angiogenic factor increased by 2 times, of which Shiquanbutu group IL-8, Leptin low expression In the control group (P <0.05). Conclusion: Shiquan Dabu Tang can improve early postoperative patients with qi and blood deficiency symptoms, shorten the drainage time and reduce the drainage area; incision drainage can promote the proliferation, migration and invasion of MDA-MB-231 Shiquan Dabu Decoction could further inhibit the proliferation, migration and invasion of MDA-MB-231 cells by decreasing the expression of IL-8, Le Ptin and other angiogenic factors in the drainage fluid.
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