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目的 探讨前列腺癌组织中端粒酶hTRT基因的表达及意义。 方法 应用原位分子杂交技术 ,对 47例前列腺癌 (PCa)、2 1例良性前列腺增生 (BPH)和 10例正常前列腺 (NP)组织中端粒酶hTRT基因进行检测和定位 ,并运用图像分析系统对hTRT表达水平与PCa分化程度的相关性进行研究。 结果 端粒酶hTRT基因在PCa中表达阳性率为 93.6 % ,表达强度与肿瘤细胞的分化程度显著相关 :未分化腺癌 >低分化腺癌 >中分化腺癌 >高分化腺癌 ;癌旁组织中hTRT基因表达率为 8.5 % ,在肿瘤组织和癌旁组织中差别有极显著性意义 (P <0 .0 1)。端粒酶hTRT基因表达水平与肿瘤细胞的分布定位一致 ,NP组织及BPH组织中端粒酶hTRT基因表达均为阴性。 结论 原位杂交检测端粒酶hTRT基因对PCa细胞水平的定位诊断具有重要意义 ,端粒酶hTRT有可能成为PCa诊断的新标志物及治疗新靶点
Objective To investigate the expression of telomerase hTRT gene in prostate cancer and its significance. Methods The hTRT gene was detected and located in 47 cases of prostate cancer (PCa), 21 benign prostatic hyperplasia (BPH) and 10 cases of normal prostatic (NP) tissues by using in situ hybridization. The relationship between hTRT expression and PCa differentiation was systematically investigated. Results The positive rate of telomerase hTRT gene expression in PCa was 93.6%. The expression intensity of hTRT gene was significantly correlated with the differentiation degree of tumor cells: undifferentiated adenocarcinoma> poorly differentiated adenocarcinoma> moderately differentiated adenocarcinoma> well-differentiated adenocarcinoma; The hTRT gene expression rate was 8.5% in the tumor tissue and paracancerous tissue was significantly different (P <0. 01). Telomerase hTRT gene expression was consistent with the distribution of tumor cells, hTRT gene expression in both NP and BPH tissues were negative. Conclusion The in situ hybridization detection of telomerase hTRT gene plays an important role in the localization diagnosis of PCa cells. Telomerase hTRT may be a new marker of PCa diagnosis and a new therapeutic target