论文部分内容阅读
目的研究肝癌组织基质金属蛋白酶组织抑制因子-1(TI MP-1)的表达,探讨其与肝癌的关系及其临床意义,为临床上寻找肝癌表达的新的生物学标志物提供理论依据。方法取山东省昌邑市人民医院2008年6月—2010年6月肝癌手术切除标本36例,按照Edmondson-Steiner组织学分级标准进行分级。选用3例外伤性肝破裂患者手术肝切除标本作正常对照。使用鼠抗人TI MP-1多克隆抗体,进行免疫组织化学PV-9000通用型二步法染色。染色结果根据反应强度及面积判断,评分标准按Shi mizu方法,根据两项打分之和判断。结果对照组中TI MP-1的表达为阴性。TI MP-1在肝癌中的表达阳性率为74.8%。TI MP-1的阳性表达与患者年龄、性别、甲胎蛋白(AFP)是否阳性、乙型肝炎表面抗原(HBsAg)是否阳性均无关(均P>0.05)。有癌栓组、无癌栓组和包膜不完整组、包膜完整组的TI MP-1在各组间差异均无统计学意义(均P>0.05)。高侵袭转移组与低侵袭转移组间,TI MP-1阳性率分别为89.6%和48.3%,组间差异有统计学意义(P<0.05)。结论 TI MP-1在肝癌组织中的过度表达是肝癌侵袭与转移程度的一个重要生物学标志物。
Objective To investigate the expression of TI MP-1 in hepatocellular carcinoma (HCC) and its relationship with hepatocellular carcinoma (HCC) and its clinical significance, and to provide a theoretical basis for searching new biomarkers for HCC expression in clinic. Methods Changyi People’s Hospital of Shandong Province from June 2008 to June 2010, 36 cases of liver cancer resection specimens were graded according to Edmondson-Steiner histological grading standards. Three patients with traumatic liver rupture were selected as normal control. The mouse anti-human TI MP-1 polyclonal antibody was used for immunohistochemistry PV-9000 universal two-step staining. Dyeing results according to the reaction intensity and area to determine the standard score Shi Shi mizu method, based on the sum of two scores to judge. Results The expression of TI MP-1 in the control group was negative. The positive rate of TI MP-1 in hepatocellular carcinoma was 74.8%. The positive expression of TI-MP-1 was not related to the patient’s age, sex, AFP, positive HBsAg (all P> 0.05). There was no significant difference between the three groups (P> 0.05). There was no significant difference in TI MP-1 among the groups with tumor embolus, tumor-free embolus group and incomplete capsule group. The positive rate of TI-MP-1 was 89.6% and 48.3% respectively between the high invasion and low invasion metastasis groups, with significant difference between the two groups (P <0.05). Conclusion The overexpression of TI-MP-1 in hepatocellular carcinoma is an important biomarker of the degree of invasion and metastasis of hepatocellular carcinoma.