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目的探讨术前区域化疗对胃癌局部淋巴结转移癌细胞凋亡及增殖的影响。方法应用原位末端标记法(TUNEL)及免疫组化技术对21例经术前区域化疗的进展期胃癌的局部淋巴结转移癌进行凋亡及细胞增殖的检测,并以未经治疗的22例进展期胃癌的局部淋巴结转移癌作为对照。结果对照组淋巴结转移癌细胞的凋亡率为6.4%±3.5%,而术前区域化疗组的细胞凋亡率16.6%±8.4%,两者差异有统计学意义(P<0.05)。PCNA的阳性率对照中为86.4.%(19/22),而术前区域化疗组中其阳性率为42.1%(8/19)。结论术前区域化疗可促进胃癌局部淋巴结转移癌细胞凋亡并抑制其增殖,术前区域化疗可作为一种有效的辅助化疗手段。
Objective To investigate the effect of preoperative regional chemotherapy on the apoptosis and proliferation of lymph node metastasis of gastric cancer. Methods TUNEL and immunohistochemistry were used to detect apoptosis and cell proliferation in 21 cases of locally advanced lymph node metastasis of advanced gastric cancer treated with preoperative regional chemotherapy. Twenty-two cases of untreated gastric cancer Local lymph node metastasis of gastric cancer as a control. Results The apoptosis rate of lymph node metastasis cells in control group was 6.4% ± 3.5%, while the apoptosis rate in preoperative chemotherapy group was 16.6% ± 8.4%. The difference was statistically significant (P <0.05). The positive rate of PCNA was 86.4% (19/22) in the control group, while the positive rate of PCNA in the preoperative chemotherapy group was 42.1% (8/19). Conclusion Preoperative regional chemotherapy can promote the apoptosis of gastric cancer with local lymph node metastasis and inhibit its proliferation. Preoperative regional chemotherapy can be used as an effective adjuvant chemotherapy.