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目的研究食管癌患者肿瘤组织和正常食管组织中脆性组氨酸三联体(fragile histidine triad,FHIT)基因的不同表达水平,探讨FHIT基因在食管癌发生发展中的作用,为食管癌的早期诊断、综合治疗和预后判断提供新的途径。方法选择因食管癌行根治切除的病人50例,将肿瘤组织和瘤旁正常组织分为试验组和对照组,应用免疫组织化学染色法标记肿瘤组织和食管正常组织中FHIT蛋白不同表达水平。应用SPSS10.0统计软件进行数据处理,得出结论。结果FHIT基因表达下降与食管癌的发生发展有关,癌组织与癌旁正常组织,FHIT基因表达下降程度有显著差异(P<0.01)。结论我们应用免疫组化方法检测了50例完成5年随访的食管癌患者的肿瘤组织和瘤旁正常组织中FHIT基因表达水平,并比较癌组织与正常组织中FHIT基因的不同表达水平,证实FHIT基因表达水平随着食管癌的进展呈显著下降趋势,证实该基因是一种抑癌基因,其表达下降与食管癌的发生发展密切相关。FHIT基因的检测对食管癌的诊断、治疗和预后判断等方面具有临床意义。
Objective To investigate the expression of fragile histidine triad (FHIT) gene in esophageal cancer tissues and normal esophageal tissues, and to explore the role of FHIT gene in the development and progression of esophageal cancer, Comprehensive treatment and prognosis provide a new way to judge. Methods Fifty patients undergoing radical resection of esophageal cancer were selected. Tumor tissues and adjacent normal tissues were divided into experimental group and control group. The expression of FHIT protein in tumor tissues and normal esophageal tissues was detected by immunohistochemical staining. Application SPSS10.0 statistical software for data processing, concluded. Results The decreased expression of FHIT gene was associated with the occurrence and development of esophageal cancer. There was a significant difference in the expression of FHIT gene between the cancerous tissues and adjacent normal tissues (P <0.01). Conclusions We used immunohistochemistry to detect the expression of FHIT gene in 50 cases of esophageal cancer patients and 5 cases of normal tissue adjacent to the tumor tissues, and compared the different expression levels of FHIT gene in cancer tissues and normal tissues. It was confirmed that FHIT The level of gene expression showed a significant decrease with the progress of esophageal cancer, confirming that the gene is a tumor suppressor gene, the expression of which is closely related to the occurrence and development of esophageal cancer. FHIT gene detection of esophageal cancer diagnosis, treatment and prognosis of clinical significance.