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目的 探讨细胞凋亡在反复高 +GZ暴露所致脑损伤中的病理作用以及碱性成纤维细胞生长因子 (b FGF)和丹参对反复高 +GZ暴露致脑损伤的防护作用及其机制。 方法 2 0只 SD大鼠随机分成 5组 ,对照组大鼠 G值为 +1GZ,实验组大鼠在动物离心机上经历了 3次 +14GZ/4 5 s(两次间间隔 30 m in)作用。 b FGF处理组、丹参处理组和生理盐水处理组在 +GZ暴露前后分别给予腹腔注射 b FGF(10 0 μg/kg)、丹参 (15 g/kg)或生理盐水 (2 m l)各一次。于暴露后 6 h处死大鼠取脑 ,用半定量反转录聚合酶链反应 (RT- PCR)方法检测大鼠脑组织中 bcl- 2和 p5 3m RNA表达以及用原位末端脱氧核苷酸转移酶 (Td T)标记法 (TUNEL )检测脑内神经元细胞凋亡情况。 结果 +GZ暴露组可见明显的 bcl- 2、p5 3m RNA表达变化和部分神经细胞凋亡 ,而 b FGF和丹参能阻断 bcl- 2和 p5 3表达变化和抑制神经细胞的凋亡。 结论 +GZ重复暴露可引起大鼠脑神经细胞凋亡及凋亡相关基因 bcl- 2和 p5 3的表达变化 ,细胞凋亡是反复高 +GZ暴露致脑损伤的机制之一 ,而 b FGF和丹参对 +GZ暴露所致的脑损伤具有防护作用。
Objective To investigate the pathological role of apoptosis in brain injury induced by repeated high + GZ exposure and the protective effect of basic fibroblast growth factor (b FGF) and salvia miltiorrhiza on brain injury induced by repeated high + GZ exposure. Methods Twenty SD rats were randomly divided into five groups. The G group in the control group was + 1GZ. The rats in the experimental group experienced three times + 14GZ / 45s on the animal centrifuge (twice in 30min intervals) . b FGF treatment group, Salvia miltiorrhiza treatment group and saline treatment group were intraperitoneally injected with bFGF (10 μg / kg), Salvia miltiorrhiza (15 g / kg) or normal saline (2 ml) respectively before and after + GZ exposure. Rats were sacrificed 6 h after exposure to take brain, bcl-2 and p5 3m RNA expression in rat brain was detected by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) TUNEL assay was used to detect neuronal apoptosis in the brain. Results The expression of bcl-2, p5 3m RNA and some neuronal apoptosis were significantly increased in + GZ exposure group, while bFGF and Salvia miltiorrhiza could block the expression of bcl-2 and p5 3 and inhibit the apoptosis of nerve cells. Conclusions + GZ repeated exposure can induce neuronal apoptosis in rat brain and the expression changes of apoptosis-related genes bcl-2 and p5 3. Apoptosis is one of the mechanisms of repeated high + GZ exposure induced brain injury, while b FGF and Salvia has a protective effect on brain damage caused by + GZ exposure.