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目的 研究人乳腺癌异种移植的自发转移、遗传稳定性和微转移的检测方法。方法 将人乳腺癌新鲜组织移植于裸鼠并连续传代 ,移植瘤细胞体外培养、再移植入裸鼠并在三个微卫星位点分析人乳腺癌、裸鼠移植瘤及其转移灶的微卫星DNA。结果 原位移植的成瘤率为 88 6 %(31/ 35 )、转移率为 4 1 9%(13/ 31)。移植瘤细胞体外培养成功 ,其再移植裸鼠成瘤率和转移率均达 10 0 %,裸鼠移植瘤及其转移灶在三个位点与人乳腺癌具有相同的微卫星DNA。结论 完整组织原位移植法可提高人乳腺癌异种移植的成功率和转移率。异种移植瘤及其传代肿瘤保持遗传稳定性。微卫星DNA鉴定法可以检测裸鼠模型的微转移。
Objective To study the detection of spontaneous metastasis, genetic stability and micrometastasis in human breast cancer xenografts. Methods Fresh human breast cancer cells were transplanted in nude mice and passaged consecutively. The transplanted tumor cells were cultured in vitro and transplanted into nude mice. The microsatellites of human breast cancer, nude mice and their metastases were analyzed at three microsatellite loci DNA. Results The tumorigenic rate of orthotopic transplantation was 88 6% (31/35) and 41 9% (13/31). The transplanted tumor cells were successfully cultured in vitro. The rate of tumorigenesis and metastasis of the transplanted tumor in nude mice reached 100%. The nude mouse xenografts and their metastases had the same microsatellite DNA with human breast cancer at three sites. Conclusion Totally orthotopic transplantation of human breast cancer can improve the success rate and metastasis rate of human breast cancer xenografts. Xenografts and their passage tumors maintain genetic stability. Microsatellite DNA identification can detect micrometastases in nude mice.