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To analyze the binding of glycyrrhetic acid (GA) to angiotensin II type I (AT1) receptor and to explore the mechanisms underlying the binding, primary cell culture of rat vascular smooth muscle cell (VSMC), radioactive ligand-receptor binding assay, lascer confocal scanning micro-scope (LCSM), Northern blot, 3H-TdR incorporation DNA assay were used in this study. The re-sults suggest that specific binding of GA to AT1 receptor (IC50 value was 35.0 mmol/L) increases intracellular [Ca2+]i of VSMC, activates transcription factor c-myc and promotes the proliferation of VSMC, therefore GA was probably an agonist of AT1 receptor, providing a new target for GA抯 pharmaceutical effects.
To analyze the binding of glycyrrhetic acid (GA) to angiotensin II type I (AT1) receptor and to explore the mechanisms underlying the binding, primary cell culture of rat vascular smooth muscle cell (VSMC), radioactive ligand-receptor binding assay, lascer confocal The re-sults suggest that specific binding of GA to AT1 receptor (IC50 value was 35.0 mmol / L) increases intracellular [Ca 2+] i of VSMC, activates transcription factor c-myc and promotes the proliferation of VSMC, therefore GA was probably an agonist of AT1 receptor, providing a new target for GA’s pharmaceutical effects.