目的 :探讨硫代磷酸化修饰的c -myc反义寡核苷酸 (ASODN)对涎腺黏液表皮样癌MEC -1细胞增殖活性的影响。方法 :c -mycASODN作用于黏液表皮样癌MEC -1细胞 ,分别采用软琼脂克隆形成试验、3 H -TdR掺入试验、裸鼠成瘤能力观察等方法研究对细胞增殖活性的影响。结果 :c -mycASODN作用于黏液表皮样癌MEC -1细胞后 ,细胞在软琼脂内的克隆形成与对照组比较明显降低 (P <0 0 1) ,其克隆形成能力受到明显的影响。3 H -TdR掺入试验显示 ,3 H -TdR掺入抑制率升高 ,c -mycASODN使MEC -1细胞DNA的合成受到显著抑制 ,且表现出浓度依赖性。用ASODN处理后的细胞接种于裸鼠后 ,细胞在裸鼠体内成瘤数量减少 ,肿瘤生长减慢。结论 :经c -mycASODN作用后 ,黏液表皮样癌MEC -1细胞的增殖受到明显的抑制 ,细胞的恶性表型得到了一定程度的逆转。 AIM: To investigate the effect of ASODN on the proliferation of mucoepidermoid carcinoma MEC-1 cells in salivary glands. Methods: The effect of c-myc ASODN on mucoepidermoid carcinoma MEC-1 cells was studied by using soft agar colony formation assay, 3H-TdR incorporation assay and tumorigenesis ability of nude mice. Results: After c-myc ASODN was applied to mucoepidermoid carcinoma MEC-1 cells, the colony formation in soft agar was significantly decreased compared with the control group (P <0.01), and its clonogenic capacity was significantly affected. 3 H -TdR incorporation assay showed that inhibition of 3 H -TdR incorporation was increased, and c-myc ASODN significantly inhibited the DNA synthesis of MEC-1 cells in a concentration-dependent manner. After ASODN-treated cells were seeded into nude mice, the number of tumor cells in nude mice was reduced and tumor growth slowed down. Conclusion: The proliferation of mucoepidermoid carcinoma MEC-1 cells was significantly inhibited by c-myc ASODN, and the malignant phenotype of cells was reversed to a certain extent.