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目的探讨大鼠重症急性胰腺炎(SAP)T淋巴细胞表型的改变及沙利度胺(反应停)对其的影响。方法 54只SD大鼠随机分成模型组(S组)、干预组(T组)和假手术组(C组),每组18只,采用5%牛磺胆酸钠胰胆管逆行注射方法建立SAP动物模型,SAP建模1h后用沙利度胺200mg/kg灌胃作为T组,开腹翻动胰腺后随即关腹作为C组。各组分术后3、6、12h3个时间点,处死大鼠收集外周血,利用流式细胞术检测外周血CD4+、CD8+、CD4+CD25+及CD3+-ICOS+的T细胞水平变化,对胰腺行常规病理学检测并评分。结果 (1)S组和C组相比,CD4+T细胞在3、6h明显增高,12h明显降低(均P<0.05);CD8+T细胞在6、12h明显下降(均P<0.05);CD4+CD25+T细胞在3、6、12h明显下降(均P<0.05);CD3+-ICOS+T细胞在6、12h明显下降(均P<0.05)。(2)T组和S组相比,CD4+T细胞在6h明显降低,12h明显增高(均P<0.05);CD8+T细胞在12h明显增高(P<0.05);CD4+CD25+T细胞有增高趋势,但差异无统计学意义(P>0.05),CD3+-ICOS+T细胞在12h明显增加(P<0.05)。(3)在3个时间点时S组的胰腺病理评分均较C组明显增高,T组较S组明显降低(均P<0.05)。结论沙利度胺的干预具有改善SAP时免疫紊乱的作用。
Objective To investigate the changes of T lymphocyte phenotypes in rats with severe acute pancreatitis (SAP) and the effects of thalidomide (response stop) on it. Methods Fifty-four SD rats were randomly divided into model group (S group), intervention group (T group) and sham operation group (C group), with 18 rats in each group. SAP was established by retrograde injection of sodium taurocholate Animal models, SAP modeling 1h thalidomide 200mg / kg gavage as T group, open the pancreas immediately after closing the abdomen as C group. Peripheral blood was collected at 3, 6, and 12 hours after operation in all groups. The levels of CD4 +, CD8 +, CD4 + CD25 + and CD3 + -ICOS + T cells in peripheral blood were detected by flow cytometry. Pathological examination and score. Results (1) Compared with group C, CD4 + T cells increased significantly at 3 and 6h and decreased significantly at 12h (P <0.05). CD8 + T cells decreased significantly at 6 and 12h (all P <0.05) CD4 + CD25 + T cells decreased significantly at 3,6,12h (all P <0.05); CD3 + -ICOS + T cells decreased significantly at 6 and 12h (all P <0.05). (2) Compared with S group, CD4 + T cells decreased significantly at 6h and increased significantly at 12h (P <0.05); CD8 + T cells increased significantly at 12h (P <0.05); CD4 + CD25 + T cells (P> 0.05). CD3 + -ICOS + T cells increased significantly at 12h (P <0.05). (3) At 3 time points, the pathological score of pancreatic tissue in group S was significantly higher than that in group C, while it was lower in group T than that in group S (all P <0.05). Conclusion Thalidomide intervention has the effect of improving immune disorder in SAP.