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目的探讨胆固醇酯转运蛋白(cholesteryl ester transfer protein,CETP)基因TaqⅠ酶切多态性与维吾尔族2型糖尿病(T2DM)及其体内脂质谱的关系。方法依据病例对照设计方法,采用全自动生化分析仪等技术测地尔族体内脂质谱;应用聚合酶链反应-限制性内切酶长度多态性(PCR-RFLP)方法,对维吾尔族的53例T2DM患者、53例糖耐量异常患者和106名糖耐量正常者的CETP基因TaqⅠ酶切多态性进行比较分析。结果3组人群空腹血糖(FPG),正常组为(5.14±0.24)mmol/L,T2DM组为(10.79±3.34)mmol/L,糖耐量异常组为(5.97±0.41)mmol/L(F=205.87,P=0.000);CETP基因型在3组人群中的分布差异有统计学意义(P=0.028);T2DM组、正常组、异常组的H+H-基因型频率分别为69.8%,45.3%,41.5%;T2DM组明显高于其他2组;不同基因型的空腹血糖(FPG),H-H-为(6.04±1.72)mmol/L,H+H+为(6.03±1.63)mmol/L,H+H-为(7.48±3.60)mmol/L,差异有统计学意义(P=0.001)。对T2DM组和正常组进行Logistic回归分析显示,T2DM的独立危险因素依次是甘油三酯(TG)(P=0.000)、CETP基因型(P=0.007)和腰围(P=0.048),H+H-基因型患T2DN的危险性是H-H-的4.042倍(95%CI=1.243~13.149,P=0.020)。结论CETP基因TaqⅠ酶切多态性与新疆维吾尔族T2DM的危险性密切相关,H+H-基因型可能是T2DM的遗传危险因素。
Objective To investigate the relationship between Taq Ⅰ polymorphism of cholesteryl ester transfer protein (CETP) gene and type 2 diabetes mellitus (T2DM) in vivo and its lipid profile in vivo. Methods According to the case-control design method, the lipid profile in the Diur population was determined by automatic biochemical analyzer. The PCR-RFLP method was used to detect the lipid profile of Uighur population In the case of T2DM, 53 patients with impaired glucose tolerance and 106 normal glucose tolerance patients were analyzed by Taq Ⅰ polymorphism of CETP gene. Results Fasting plasma glucose (FPG) was (5.14 ± 0.24) mmol / L in normal group, (10.79 ± 3.34) mmol / L in T2DM group and (5.97 ± 0.41) mmol / L in normal glucose tolerance group 205.87, P = 0.000). The distributions of CETP genotypes in three groups were statistically different (P = 0.028). The frequencies of H + H genotypes in T2DM group, normal group and abnormal group were 69.8% and 45.3 % And 41.5%, respectively; T2DM group was significantly higher than the other two groups; fasting blood glucose (FPG), HH was (6.04 ± 1.72) mmol / L and H + H + was 6.03 ± 1.63 mmol / L + H- was (7.48 ± 3.60) mmol / L, the difference was statistically significant (P = 0.001). Logistic regression analysis showed that the independent risk factors of T2DM were triglyceride (TG) (P = 0.000), CETP genotype (P = 0.007), waist circumference (P = 0.048), H + H - Genotype T2DM risk is 4.042 times that of HH- (95% CI = 1.243-13.149, P = 0.020). Conclusion The Taq Ⅰ polymorphism of CETP gene is closely related to the risk of T2DM in Uighur Xinjiang. H + H-genotype may be the genetic risk factor of T2DM.