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目的：通过对临床科室多药耐药菌（ MDROs）目标性监测，了解MDROs分布，评价干预效果，为临床有效控制MDROs感染提供依据。方法2013年1月－2014年7月对137例MDROs感染病例进行目标性监测与实施干预，每天通过LIS系统监测全院MDROs，并到科室督查实施。结果分离出病原菌759株，其中多药耐药菌株137株，占18．05％；主要来自痰，占31．39％；大肠埃希菌居第1位，占44．53％；ICU是MDROs主要来源；单间安置率33．58％，隔离标识使用率66．42％，手卫生依从性45．99％，无菌操作98．54％；137例患者中，治愈53例（38．69％），好转6例（4．38％），未愈68例（49．64％），自动出院9例（6．57％），死亡1例（0．73％）。结论在MDROs预防控制中，重点关注ICU及大肠埃希菌感染预防控制工作，MDROs未愈率较高，治疗效果差，临床控制力度还需进一步加强。“,”Objective To conduct the multi-drug resistant bacteria ( MDROs) targeted surveillance of the clinical de-partments ,understand MDROs distribution , evaluate the intervention effect , providing the basis for clinical effective control MDROs infection .Methods Conducted targeted MDROs monitoring and intervention for 137 cases of patients with infection from January 2013 to July 2014,monitored daily hospital MDROs through LIS system to supervise the implementation of the de-partment.Results 759 strains pathogenic bacteria was separated ,including multidrug-resistant strains of 137,accounting for 18.05%;mainly from sputum,accounting for 31.39%;E.coli bacteria ranked first ,accounting for 44.53%;ICU was the major source of MDROs;single room placement rate 33.58%,utilization rate of isolation identified was 66.42%,compliance with hand hygiene 45.99%,aseptic 98.54%;Among 137 cases of patients,53 cases(38.69%) was cured,improved in 6 cases (4.38%),cured 68 cases(49.64%),discharged 9 cases(6.57%),died 1 case(0.73%).Conclusion In the MDROs pre-vention and control ,focus on ICU and E .coli infection prevention and control ,the rate of MDROs not cured is higher ,the treat-ment is poor,clinical control efforts need to be further strengthened .