肝细胞培养是研究肝脏的重要方法之一。其实验因素单纯易于分析,经济迅速,可进行人体上不便进行的实验。目前国内多采用鼠肝细胞,有种属差异问题,采用人肝或人胎肝细胞则更为理想。白蛋白是公认的由肝实质细胞特异分泌的蛋白,用放射免疫方法测定培养肝细胞白蛋白分泌量及其变化是特异地反映培养肝细胞功能的较好指标。为此,我们建立了一种较国外简单可靠的放射免疫方法。 Liver cell culture is one of the important ways to study the liver. Its simple and easy to analyze experimental factors, rapid economy, the inconvenience of human experiments. At present, the domestic use of mouse hepatocytes, there is a problem of species differences, the use of human liver or human fetal hepatocytes is even more ideal. Albumin is a recognized protein specifically secreted by liver parenchymal cells. The radioimmunoassay of albumin in cultured hepatocytes is a good indicator of the function of cultured hepatocytes. To this end, we have established a simple and reliable method of radioimmunoassay abroad.