论文部分内容阅读
目的:观察糖足福对糖尿病足部溃疡的治疗作用。方法:雄性SD大鼠90只,随机取10只作为正常对照组,其余大鼠以高糖高脂饲料加链脲佐菌素制备大鼠2型糖尿病模型,再以足部皮肤切割法制备足部溃疡模型。将模型大鼠随机分为模型对照组、糖足福6.25g/100ml、3.13g/100ml、1.56g/100ml组、二甲双胍100 mg/kg联合糖足福6.25g/100ml组。采用35~38℃药液浸泡溃疡足,模型对照组以20%的乙醇浸泡,每次30min,每天1次,直至溃疡痊愈。记录各组大鼠溃疡痊愈时间,检测血糖、血清胰岛素(INS)、血脂水平,计算胰岛素抵抗指数(IRI)和胰岛素敏感指数(ISI)。结果:糖足福6.25g/100ml、3.13g/100ml、1.56g/100ml组大鼠足部溃疡的愈合时间均显著小于模型对照组、血糖水平均显著低于模型对照组、IRI均明显著低于模型对照组、ISI均高于模型对照组、大鼠进食量及饮水量均显著低于模型对照组。糖足福6.25g/100ml组INS水平低于模型对照组。糖足福6.25g/100ml、3.13g/100ml、1.56g/100ml组大鼠血清中TG、TC、HDL-C和LDL-C含量与模型对照组比较差异无统计学意义。结论:糖足福有相对较弱的提高岛素敏感性和降低血糖作用,但其具有明显的促进糖尿病大鼠足部溃疡愈合作用,说明其促进糖尿病足部溃疡愈合作用主要通过局部作用而产生的。
Objective: To observe the therapeutic effect of Tang Zu Fu on diabetic foot ulcer. Methods: Ninety male Sprague-Dawley rats were randomly divided into normal control group (n = 10) and other rats were given high-sugar and high-fat diet plus streptozotocin to make type 2 diabetic rat model. Ulcer model. The model rats were randomly divided into model control group, Tangfushan 6.25g / 100ml, 3.13g / 100ml, 1.56g / 100ml group, metformin 100 mg / kg and Tangzuofu 6.25g / 100ml group. Using 35 ~ 38 ℃ soak ulcer foot solution, the model control group with 20% ethanol soaked every 30min, day 1, until the ulcer healed. The healing time of ulcer in each group was recorded. The levels of blood glucose, serum insulin (INS) and blood lipids were measured. Insulin resistance index (IRI) and insulin sensitivity index (ISI) were calculated. Results: The healing time of foot ulcer in 6.25g / 100ml, 3.13g / 100ml and 1.56g / 100ml group was significantly less than that of the model control group, the blood glucose level was significantly lower than the model control group, IRI were significantly lower In the model control group, ISI were higher than the model control group, the food intake and water intake of rats were significantly lower than the model control group. The level of INS in 6.25g / 100ml group was lower than that in model control group. The content of TG, TC, HDL-C and LDL-C in 672g / 100ml, 1.56g / 100ml group were not significantly different from the model control group. CONCLUSION: Tangfufu has relatively weak insulin sensitivity and hypoglycemic effect, but it has a significant promotion of foot ulcer healing in diabetic rats, indicating that its promotion of diabetic foot ulcer healing occurs mainly through local action of.