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尿胆原由小肠和大肠远端的微生物作用于胆红素而生成。是无色的开环四吡咯化合物,很不稳定,易脱氢生成橙黄色的尿胆素。尿胆素又可还原为尿胆原。尿胆原又可与酸性对二甲基氨基苯甲醛(Ehrlich’s 试剂)反应生成红色复合物。此外,尿胆素可与锌生成螯合物,产生特种绿色荧光(Sc-hlesinger 反应)。Schlesinger 氏法常用于定性,由于个别偏差不适于定量。本文改良了Schlesinger 氏法,可作简单、快速而精确的测定。材料和方法各种溶:剂和试剂均用分析级配制。已知含量尿胆原按Henry 法制备:用0.72mol/
Urobilinogen from the small intestine and the distal colon of the role of microorganisms in the formation of bilirubin. Is a colorless, open-loop tetrapyrrole compound, is very unstable, easy to dehydrogenate to produce orange-yellow Urobilinogen. Urobilinogen can be reduced to urinary gallstones. Urobilinogen, in turn, reacts with acidic p-dimethylaminobenzaldehyde (Ehrlich’s reagent) to form a red complex. In addition, urobilin can form chelates with zinc and produce special green fluorescence (Sc-hlesinger reaction). Schlesinger’s method is often used for qualitative, because individual deviation is not suitable for quantitative. In this paper, Schlesinger’s method is improved, which can be used for simple, rapid and accurate determination. Materials and Methods Various Solvents: The reagents and reagents are prepared using analytical grade. The known content of urinary gallstones was prepared by Henry method: with 0.72mol /