本文介绍一种适于制作各种小血管的扫描电镜样品制备方法,操作简便,效果良好。此法是经血管灌注后,将所取冠状动脉组织经电镜样品常规制作,干燥后,用Aldite包埋剂部分包埋,然后固定在电镜样品托上,纵行剖开,喷金后观察。此方法可防止血管内膜的人工损伤,使观察结果更具有真实性,效果较好。 This article describes a scanning electron microscopy sample preparation method suitable for making various small blood vessels. The operation is simple and the effect is good. This method is through the blood vessels after perfusion, the coronary tissue obtained by electron microscopy samples of conventional production, drying, embedding with Aldite embedding agent, and then fixed in the electron microscope sample holder, longitudinal split, gold spray observed. This method can prevent the artificial damage of the intima of the blood vessel, so that the observation result is more authentic and the effect is better.