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目的研究人参皂苷Rg1对铅引起的大鼠海马CA1区长时程增强(LTP)的损伤是否有修复作用。方法 Wistar大鼠从出生至断奶通过母乳摄入铅(母鼠每天饮用0.2%醋酸铅溶液20 mL),在出生后的20 ～25 d记录其海马CA1区兴奋性突触后电位并诱导长时程增强。结果人参皂苷Rg1 (50μmo.lL-1)灌流对照组和铅暴露组大鼠海马脑片20min均能诱导出LTP,铅暴露组大鼠的LTP幅度较对照组低。在铅暴露组大鼠的海马脑片上,高频刺激(HFS,1 s, 100 Hz)诱导的LTP较对照组显著降低, 50μmol.L-1人参皂苷Rg1灌流20 min,HFS诱导的LTP幅度提高47.1%。结论人参皂苷Rg1能够提高基础的突触传递,并能部分修复铅损伤的HFS-LTP。
Objective To study whether ginsenoside Rg1 can repair long-term potentiation (LTP) injury induced by lead in hippocampal CA1 region of rats. METHODS: Wistar rats were exposed to breast milk from birth to weaning (adults consumed 20 mL of 0.2% lead acetate solution per day) and recorded excitatory postsynaptic potentials and induced long-term hippocampal CA1 region from day 20 to day 25 after birth. Enhanced. Results LTP was induced by ginsenoside Rg1 (50μmol·lL-1) perfusion control group and lead-exposed rats hippocampus for 20 min. The LTP amplitude of lead exposure group was lower than that of control group. HTP induced by high-frequency stimulation (HFS, 1 s, 100 Hz) was significantly decreased in hippocampal slices of rats exposed to lead compared with the control group. Perfusion of 50 μmol.L-1 ginsenoside Rg1 for 20 min increased the amplitude of LTP-induced LTP. 47.1%. Conclusion Ginsenoside Rg1 can increase the basal synaptic transmission and partially repair lead-damaged HFS-LTP.