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目的:了解感染所引起的新生大鼠感染性脑组织中Sox17和(髓鞘碱性磷酸酶)MBP的表达。方法:将64只2日龄SD大鼠随机分为对照组、实验组。实验组生后第2~6 d持续腹腔注射脂多糖(LPS)每日0.6 mg/kg(0.01 rnl/g)。对照组则按照相同的注射方式及注射剂量,给予生理盐水,建立新生大鼠脑白质损伤模型。注射后按观察时间点不同分为1 d、3 d、7 d、15 d,4个亚组(n=8)。处死大鼠取出脑白质,HE染色观察脑白质区病理变化,Western Blot法测定各组脑组织中Sox17和MBP蛋白的表达,RT-RCR法检测Sox17和MBPmRNA的表达变化。结果:实验组大鼠生长发育缓慢,HE染色显示脑白质区出现组织水肿,部分核固缩、核碎裂。PCR和Western Blotting结果发现Sox17在生后1 d开始表达,7 d达高峰,随后下降,各时间点实验组与对照组相比差异有统计学意义(P<0.05)。MBP在实验组的表达显著下降,各时间点实验组与对照组相比差异有统计学意义(P<0.05)。结论:生后感染可导致新生大鼠脑白质损伤,MBP表达减少,Sox17表达明显升高,提示Sox17剂量的升高对感染后新生大鼠脑组织可能具有保护性修复作用。
Objective: To investigate the expression of Sox17 and myelin alkaline phosphatase (MBP) in infectious brain tissue of neonatal rats induced by infection. Methods: 64 2-day-old SD rats were randomly divided into control group and experimental group. The rats in experiment group were injected intraperitoneally with lipopolysaccharide (LPS) 0.6 mg / kg (0.01 rnl / g) on the 2nd to 6th day after birth. Control group according to the same injection and injection dose, given saline, the establishment of neonatal rat model of white matter damage. After injection, they were divided into 4 subgroups (n = 8) on the 1st, 3rd, 7th and 15th days according to the observation time points. The rats were sacrificed and the white matter was removed. The pathological changes of white matter were observed by HE staining. The expressions of Sox17 and MBP proteins were determined by Western Blot. The expressions of Sox17 and MBP mRNA were detected by RT-PCR. Results: The growth and development of rats in the experimental group were slow. HE staining showed tissue edema, partial nuclear condensation and nuclear fragmentation in the white matter area. PCR and Western Blotting results showed that Sox17 began to express on the 1st day after birth and peaked on the 7th day, then decreased. There was significant difference between the experimental group and the control group at each time point (P <0.05). The expression of MBP in the experimental group decreased significantly. There was significant difference between the experimental group and the control group at each time point (P <0.05). CONCLUSION: Postnatal infection can lead to white matter damage in neonatal rats, decreased expression of MBP and increased expression of Sox17, suggesting that elevated Sox17 may have a protective effect on the brain tissue of neonatal rats after infection.