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肺结核病的诊断主要是根据临床表现、胸部X线检查及痰涂片检查。胸部X线检查缺乏特异性,痰菌检查的阳性率又较低,痰培养虽可提高诊断的阳性率,但费时较长(6～8周),且阳性率仍较低(50％)。因此,探测更为简便的结核病诊断方法已引起人们普遍关注。近年来兴起的抗结核分支杆菌抗体的血清学诊断方法日益受到重视。我们采用金标免疫斑点渗滤试验(TB—DOT)快速检测结核菌IgG抗体,并与常规ELiSA法作对比观察,同时和传统PPD试验作对照,以观察其应用价值。
The diagnosis of tuberculosis is mainly based on clinical manifestations, chest X-ray examination and sputum smear examination. Chest X-ray examination of the lack of specificity, sputum examination of the positive rate and lower sputum culture may increase the diagnostic positive rate, but more time-consuming (6 to 8 weeks), and the positive rate is still low (50%). Therefore, the detection of simpler methods of diagnosis of tuberculosis has aroused widespread concern. In recent years, the rise of anti-TB Mycobacterium antibody serological diagnostic methods have received increasing attention. We used the gold standard immune spotting percolation test (TB-DOT) for the rapid detection of Mycobacterium tuberculosis IgG antibody and compared with the conventional ELiSA method for comparison, and the traditional PPD test as a control to observe its value.