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目的探索建立急性脑出血致肺损伤动物模型的方法,阐释脑源性肺损伤综合征的科学概念,为该综合征的发病机制研究和临床防治奠定基础。方法随机将72只大鼠分为对照组、模型组,2组各分6个亚组(即术前及术后1h、6h、12h、24h、48h),使用Ⅳ型胶原酶—肝素钠—生理盐水联合注射建立脑出血时脑源性肺损伤综合征模型,结合呼吸频率、血气分析、白细胞计数、肺组织含水量、肿瘤坏死因子α(TNFα)及脑、肺组织病理观察,评估该动物模型。结果该模型动物不仅存在明确稳定的脑出血,而且短时间内即出现一系列炎性反应,出现严重的的肺功能损伤和明确的肺组织病理改变。结论该模型可成功地模拟急性脑出血时脑源性肺损伤综合征的病理过程。
Objective To explore the method of establishing animal model of acute lung injury induced by acute cerebral hemorrhage and to elucidate the scientific concept of brain-derived lung injury syndrome and lay the foundation for the pathogenesis research and clinical prevention and treatment of this syndrome. Methods Totally 72 rats were randomly divided into control group, model group and control group. The rats in each group were divided into 6 subgroups (preoperative and postoperative 1h, 6h, 12h, 24h, 48h). Type Ⅳ collagenase - heparin sodium - The model of cerebral injury induced by intracerebral hemorrhage was established by injecting normal saline and cerebral hemorrhage. Combined with respiratory rate, blood gas analysis, white blood cell count, lung tissue water content, tumor necrosis factor alpha (TNFα) and brain and lung histopathology, model. Results The model animals not only had a definite and stable cerebral hemorrhage, but also showed a series of inflammatory reactions in a short time, with severe lung injury and definite lung histopathological changes. Conclusion This model can successfully simulate the pathological process of brain-derived lung injury syndrome in acute intracerebral hemorrhage.