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血管平滑肌细胞钙动力学异常引起的血管收缩,是高血压发生的先决条件。近年来为了研究平滑肌细胞外Ca~(++)的跨膜内流,使用~(45)Ca示踪技术是比较好的方法。其中“镧法”更能准确地反映细胞内Ca~(++)水平。我们根据Lewanczuk等的方法略加改动,建立了“冷镧法”测量~(45)Ca细胞摄取
Vasoconstriction caused by abnormal calcium dynamics of vascular smooth muscle cells is a prerequisite for hypertension. In recent years, in order to study the transmembrane influx of extracellular Ca ~ (++) in smooth muscle cells, the use of ~ (45) Ca tracer technology is a good method. The “lanthanum method” can more accurately reflect the level of intracellular Ca ~ (++). We modified slightly according to the method of Lewanczuk et al. And established a method called “cold lanthanum method” to measure the uptake of ~ (45) Ca cells