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为探讨一氧化氮和神经元性一氧化氮是否参与急性局灶性脑缺血再灌注的发病机理，用栓线法建立大脑中动脉阻塞模型大鼠，观察急性局灶性脑缺血再灌注损伤血浆一氧化氮含量的动态变化和神经元型一氧化氮合酶抑制剂———７硝基吲唑对再灌注期一氧化氮含量的影响．结果，脑缺血３０ｍｉｎ血浆一氧化氮含量明显升高，缺血３ｈ组一氧化氮含量下降，接近对照组；缺血３ｈ、再灌注３０ｍｉｎ组血浆一氧化氮含量再次升高，再灌注３ｈ组一氧化氮含量又下降，但仍高于对照组．７硝基吲唑（２５ｍｇ／ｋｇ）能显著抑制缺血３ｈ、再灌注３０ｍｉｎ升高的血浆一氧化氮水平．７硝基吲唑的作用可被Ｌ精氨酸（３００ｍｇ／ｋｇ）逆转，而Ｄ精氨酸（３００ｍｇ／ｋｇ）无效．
To investigate whether nitric oxide and neuronal nitric oxide are involved in the pathogenesis of acute focal cerebral ischemia-reperfusion in rats, the middle cerebral artery occlusion model rats were established by suture method and the acute focal cerebral ischemia-reperfusion The dynamic change of plasma nitric oxide and the effect of neuronal nitric oxide synthase inhibitor - 7nitroindazole on nitric oxide content during reperfusion period. Results: The content of nitric oxide in plasma was significantly increased at 30 min after cerebral ischemia and the content of nitric oxide decreased in 3 h after ischemia, which was close to the control group. After 3 h of ischemia and 30 min of reperfusion, the level of plasma nitric oxide increased again, Nitric oxide content decreased again, but still higher than the control group. 7-nitroindazole (25mg / kg) significantly inhibited plasma nitric oxide levels at 3h after ischemia and 30min after reperfusion. The effect of 7-nitroindazole can be reversed by L-arginine (300 mg / kg), whereas D-arginine (300 mg / kg) is not effective.