论文部分内容阅读
试验2002～2004年在蒲江病圃和室内进行,发现不同组合及其恢复系对稻瘟病菌确实具有不同的抗性,2004年20个主要杂交水稻恢复系接种118个稻瘟病菌株后,所有菌株均至少对5个恢复系具有致病力,不同菌株间的致病力分化,使得同一田块病菌群体获得了对其他不同品种的致病力,其中对全部参试恢复系致病菌株出现频率为11 90%,显著高于仅对一个恢复系致病的菌株频率(P 0 01),水稻多样化抗性逐步为病菌所克服。利用pot2 1和pot2 2引物,对68个对多恢1号致病菌株rep PCR扩增获得多种指纹图谱,各菌株指纹图谱与其地区和品种来源无相关性,表明对多恢1号致病的菌株是由不同遗传背景的菌株演化而来。
The experiment was conducted in Pujiang nursery and indoor from 2002 to 2004 and found that different combinations and their restorer lines did have different resistance to Magnaporthe grisea. After inoculation of 118 M. grisea strains in 20 major hybrid rice restorer lines in 2004, All of them had pathogenicity to at least five restorer lines and pathogenicity differentiation among different strains so that pathogenicity of the same field patch group to other different varieties was obtained. Was 11 90%, which was significantly higher than that of the strain that only caused one pathogen (P 0 01). The diversified resistance of rice was gradually overcome by the pathogen. Using pot2 1 and pot2 2 primers, 68 fingerprinting strains of Duochui 1 were amplified by PCR, and the fingerprints of each strain were not related to their regions and breeds. The results indicated that Duochui No.1 The strains are evolved from strains of different genetic backgrounds.