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本文用Cu~(2+)(引发氧化修饰)和脂质过氧化降解产物丙二醛(MDA)对低密度脂蛋白(LDL)进行修饰,观察了两种修饰的LDL对巨噬细胞高密度脂蛋白_3(HDL_3)结合量及细胞内胆固醇酯聚集的影响。结果说明:1.Cu~(2+)和MDA修饰的LDL都可使巨噬细胞HDL_3结合量下降,细胞内脂质过氧化物(LPO)含量升高,但当处理细胞在含10％无脂血清(LPDS)培养液中继续培养时,由MDA修饰的LDL(MDA-LDL)导致的HDL_3结合量降低又有一定的恢复,细胞内LPO含量不再升高,而Cu~(2+)修饰的LDL(Cu~(2+)-LDL)处理的细胞继续培养时,HDL_3结合量则继续下降,细胞LPO含量则继续升高。2.由Cu~(2+)-LDL导致的巨噬细胞HDL_3结合量下降与细胞LPO含量升高之间呈负相关(r=-0.81,P<0.01)。3.MDA-LDL和Cu~(2+)-LDL都可造成巨噬细胞胆固醇酯聚集,但MDA-LDL造成的胆固醇酯可被HDL_3大量清除而Cu~(2+)-LDL造成的胆固醇酯聚集则不能。
In this paper, low density lipoprotein (LDL) was modified by Cu ~ (2 +) (initiated oxidation) and malondialdehyde (MDA), the effect of two modified LDL on macrophage high density Lipoprotein_3 (HDL_3) binding capacity and the accumulation of intracellular cholesterol esters. The results showed that: 1. Both Cu 2+ and MDA-modified LDL could decrease the HDL-3 binding capacity of macrophages and increase the intracellular lipid peroxide (LPO) content. However, when treated with 10% In LPDS culture medium, the HDL-3-binding capacity of LDL (MDA-LDL) induced by MDA decreased and then recovered, while the content of LPO in cells increased no longer. When the cultured cells treated with modified LDL (Cu ~ (2 +) - LDL) continued to grow, the amount of HDL - 3 binding continued to decline, while the level of LPO in cells continued to increase. The decrease of HDL_3 binding of macrophages induced by Cu ~ (2 +) - LDL was negatively correlated with the increase of cell LPO (r = -0.81, P <0.01). Both MDA-LDL and Cu ~ (2 +) - LDL can cause cholesterol ester accumulation in macrophages, but cholesterol ester caused by MDA-LDL can be eliminated by HDL-3 and cholesterol ester Aggregation can not be.