利用基因同源重组和基因割裂的方法，研究了枯草杆菌ｋｉ－２－１３２中ｄｅｇＵＳ基因的作用。发现此基因对菌株的蛋白酶产生、感受态的形成、细胞运动、葡萄糖对产酶的抑制等系列表型都有影响，是一个多效基因。通过对此基因的割裂分析，发现单纯ｄｅｇＵＳ阅读框对菌生长时的细胞形态及表达载体上ａｐｒＥ的表达有明显的影响，而对其他表型的影响不明显 The effect of degUS gene in B. subtilis ki-2-132 was studied by homologous recombination and gene fragmentation. The gene was found to be a pleiotropic gene that affected the protease production, the formation of competent cells, cell motility, and the inhibition of glucose-producing enzymes. Through the fragmentation analysis of this gene, it was found that the pure degUS reading frame had a significant effect on the cell morphology during the growth of the bacteria and the expression of aprE on the expression vector, but not on the other phenotypes