论文部分内容阅读
根据流行于我国的两型ＨＦＲＳＶ代表株汉滩型７６１１８株及汉城型Ｒ２２株Ｍ节段的核酸序列，设计两型共同引物，建立了逆转录－聚合酶链反应（ＲＴＰＣＲ）方法，检测３９株从不同地区、不同宿主分离的ＨＦＲＳＶ感染鼠脑及细胞培养物；同时还建立了捕捉ＥＬＩＳＡ法（ｃＥＬＩＳＡ），检测了３９株中的３６株，每份样本设复孔，以Ｐ／Ｎ≥２．１０且Ｐ－Ｎ≥０．１０者判为阳性。ＲＴＰＣＲ及ｃＥＬＩＳＡ两法的检出率分别为９７．６％与８２．４％，二者符合率８４．６％。此外，对ＲＴＰＣＲ产物进行酶切分型，３８份扩增产物中的１５份可被ＡｌｕＩ切开。根据所获酶切图谱的差异，可分为汉滩型及汉城型两型，显示了酶切分型的潜在价值
According to the nucleotide sequences of strain 76 Han-118 and strain M22 of Seoul-type HFRSV, which were popular in China, two types of primers were designed and the reverse transcription-polymerase chain reaction (RT-PCR) method was established , 39 strains of HFRSV isolated from different regions and different hosts were tested for their brain and cell cultures infected with HFRSV. At the same time, a cELISA was established and 36 strains of 39 strains were detected. /N≥2.10 and P-N≥0.10 were judged as positive. The detection rates of RT-PCR and cELISA were 97.6% and 82.4% respectively, the coincidence rates of the two methods were 84.6%. In addition, RT-PCR products were digested by restriction enzyme digestion, 15 copies of 38 amplification products can be AluI incision. According to the differences of the digested maps, it can be divided into two types: Han Tan type and Seoul type, indicating the potential value of digestion and typing