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目的 探讨慢性苯中毒患者出现血象异常的机制。方法 采用末端脱氧核苷酸转移酶介导的脱氧三磷酸尿嘧啶末端标记法 (TUNEL法 )和免疫组化法分别对慢性苯中毒患者的骨髓标本进行凋亡细胞和增殖细胞核抗原 (PCNA)的检测 ,并分别与正常对照组比较 ,同时将慢性苯中毒患者的骨髓细胞凋亡指数 (AI)和增殖细胞核抗原指数 (PI)作直线相关分析 ,将AI值和PI值分别与所测血象中的白细胞计数、中性粒细胞计数、红细胞计数、血红蛋白量、血小板计数作直线相关分析。结果 慢性苯中毒患者的骨髓细胞AI值为 2 4 .7%± 12 .0 % ,高于正常对照组 (7.3%± 3.5 % ) ,差异有显著性 (P <0 .0 5 ) ;慢性苯中毒组PI值为 10 .7%± 7.8% ,明显低于正常对照组 (31.8%± 11.7% ) ,差异有显著性 (P <0 .0 1) ;慢性苯中毒组的AI值与PI值之间没有直线相关性 (r =0 .36 ,P >0 .0 5 ) ,这两者与血象中各项指标无直线相关性 (P >0 .0 5 )。结论 人类慢性苯中毒的发病过程可出现骨髓细胞凋亡及增殖失衡 ,与造血功能障碍有关。
Objective To investigate the mechanism of abnormal blood in patients with chronic benzene poisoning. Methods Apoptotic cells and proliferating cell nuclear antigen (PCNA) were detected by terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) and immunohistochemistry in bone marrow samples of patients with chronic benzene poisoning (AI) and proliferating cell nuclear antigen index (PI) in patients with chronic benzene poisoning were determined by linear correlation analysis. The AI value and PI value were respectively compared with the measured blood levels White blood cell count, neutrophil count, red blood cell count, hemoglobin, and platelet count were analyzed by linear correlation. Results The AI value of bone marrow cells in patients with chronic benzene poisoning was 24.7% ± 12.0%, which was significantly higher than that in the normal control group (7.3% ± 3.5%) (P <0.05). Chronic benzene The PI of poisoning group was 10.7% ± 7.8%, which was significantly lower than that of normal control group (31.8% ± 11.7%) (P <0.01). The AI and PI of chronic benzene poisoning group There was no linear correlation between them (r = 0.36, P> 0.05). There was no linear correlation between them (P> 0.05). Conclusion The pathogenesis of chronic benzene poisoning in human may appear imbalance of bone marrow cell apoptosis and proliferation, which is related to hematopoietic dysfunction.