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目的 探讨日本血吸虫核酸疫苗VR10 12 /Sj3 1粘膜免疫诱导小鼠抗攻击感染的保护力。 方法 将实验小鼠随机分为 5组 ,每组 12只。A组为单磷脂 (MPL)组 ,B组为VR10 12组 ,C组VR10 12 +MPL组 ,D组为VR10 12 /Sj3 1组 ,E组为VR10 12 /Sj 3 1+MPL组。滴鼻免疫。在第 3次免疫后 2周 ,每只鼠经腹部感染 40± 1条尾蚴 ,45d后宰杀 ,观察减虫率和减卵率。在初次免疫前和攻击感染前尾静脉采血并收集粪便 ,ELISA检测血清内特异性IgA、IgG及粪内sIgA水平。 结果 与对照组相比 ,VR10 12 /Sj 3 1及VR10 12 /Sj 3 1加MPL滴鼻免疫均可引起小鼠血清IgG、IgA和粪内sIgA升高 ,且VR10 12 /Sj 3 1加佐剂滴鼻免疫组抗体的增幅 >不加佐剂组。VR10 12 /Sj3 1和VR10 12 /Sj3 1加MPL滴鼻免疫诱导小鼠产生的减虫率分别为 2 1.70 %和 2 8.82 % ,减卵率分别为 2 7.98%和 40 .72 %。二者的减虫率差异无显著性 (P >0 .0 5 ) ,但减卵率后者显著高于前者 (P <0 .0 5 )。 结论 VR10 12 /Sj3 1滴鼻免疫可引起小鼠产生全身和粘膜免疫应答及部分抗攻击感染的免疫保护力。
Objective To investigate the protective effect of Schistosoma japonicum nucleic acid vaccine VR10 12 / Sj3 1 against mucosal immunity induced by anti-challenge in mice. Methods Experimental mice were randomly divided into 5 groups of 12 rats. Group A was monophospholipid (MPL), group B was VR10 12, group C VR10 12 + MPL, group D VR10 12 / Sj3 1, group E VR10 12 / Sj 3 1 + MPL. Nasal immunization. Two weeks after the third immunization, each mouse was infected with 40 ± 1 cercaria via abdomen and sacrificed 45 days later. The worm reduction rate and the oviposition rate were observed. Before the first immunization and before challenge infection, blood samples were taken from the tail vein and collected for faecal detection. The serum IgA, IgG and sIgA in feces were detected by ELISA. Results Compared with the control group, intranasal immunization with VR10 12 / Sj 3 1 and VR10 12 / Sj 3 1 plus MPL resulted in an increase in serum IgG, IgA and fecal sIgA in mice, and VR10 12 / Sj 3 1 plus adjuvant Intranasal immunization group antibody increase> without adjuvant group. The worm reduction rates of mice immunized with VR10 12 / Sj3 1 and VR10 12 / Sj3 1 plus MPL were 21.7% and 28.82%, respectively, and the rates of reducing eggs were 2 7.98% and 40.72% respectively. The worm reduction rate was not significantly different between the two groups (P> 0.05), but the latter was significantly higher than the former (P <0.05). Conclusion Intranasal immunization with VR10 12 / Sj3 1 can cause systemic and mucosal immune responses in mice and partial immunological protection against challenge infection.