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侧枝与黄瓜(Cucumis sativus)产量有密切关系.本实验利用侧枝长势较弱、萌发较早的华北类型品系S94和侧枝长势较强、萌发较晚的欧洲类型品系S06构建的224个F6:7家系进行黄瓜侧枝相关性状的研究.利用已构建的重组自交系群体遗传图谱,使用软件WinQTLCart2.5进行复合区间定位.在2006年秋和2007年春两季,共检测到4个侧枝相关性状(侧枝均长LBAL、侧枝总长LBTL、侧枝数目LBN和第一侧枝节位FLBN)的36个QTL,单个QTL的贡献率在3.1%(lbtl2.1,春季)~32.3%(lbn2.3,春季)之间.结果显示,4个不同性状的11个QTL(lbal1.1,lbtl1.1,lbn1.2,flbn1.2等)在两季中都聚集在第1连锁群e23m18d~ME23EM6c之间(7.4cM),并且在第2连锁群的S94A1~ME4SA4a之间(13.9cM)也检测到了4个不同性状的15个QTL(lbal2.1,lbtl2.1,lbn2.1和flbn2.1等).两季共有21个QTL贡献率超过10%,其中lbn2.3的贡献率(春季32.3%,LOD=18.4)为最大,lbtl1.3(秋季26.2%,LOD=17.4;春季26.9%,LOD=17.9)在两季的位置和贡献率都稳定.这些基因座为将来进行QTL精细定位提供了参考,同时利用其紧密连锁(<10cM)的特异标记(CMBR40,F,CS30,S94A1,CSWTA11B)可进行黄瓜侧枝性状的分子标记辅助育种.
The yield of cucumber (Cucumis sativus) was closely related to the growth of cucumber (Cucumis sativus) .In this study, 224 F6: 7 lines were constructed from the North China type S94 with relatively weak growth and early germination, and S06 with the stronger late- The research was carried out on the related traits of cucumber side shoots.Using the genetic map of the constructed recombinant inbred lines, software WinQTLCart2.5 was used for composite interval mapping. In autumn 2006 and spring 2007, four collateral traits (collateral 36 LBs with long LBALs, LBTLs with total length of branches, LBNs with lateral branches, and FLBNs with the first branch. The contribution rate of individual QTLs was between 3.1% (lbtl2.1, spring) and 32.3% (lbn2.3, spring) The results showed that eleven QTLs (lbal1.1, lbtl1.1, lbn1.2, flbn1.2, etc.) of four different traits clustered in the first linkage group between e23m18d and ME23EM6c (7.4cM) , And four QTLs (lbal2.1, lbtl2.1, lbn2.1 and flbn2.1, etc.) of different traits were also detected between S94A1 and ME4SA4a (13.9cM) in the second linkage group. The contribution rate of 21 QTLs was over 10%, among which the contribution rate of lbn2.3 was the highest (32.3% in spring and 18.4% in LOD), lbtl1.3 (26.2% in autumn and LOD = 17.4 in spring; 26.9% in spring and LOD = 17 .9) were stable in both seasons and contribution rate.These loci provide a reference for future fine mapping of QTLs, and utilize their specific markers (CMBR40, F, CS30, S94A1 and CSWTA11B) closely linked (<10cM) Cucumber lateral branch traits can be molecular marker assisted breeding.