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目的:制备高表达GM-CSF的C57小鼠T淋巴瘤细胞系。方法:将小鼠GM-CSF真核表达质粒用电穿孔方法导入C57小鼠T淋巴瘤细胞中,倍比稀释法制备单个细胞克隆,经RT-PCR、骨髓干细胞增殖实验和集落形成实验筛选相对高表达GM-CSF的RMA-GM克隆。结果:获得了高表达GM-CSF的RMA细胞系RMA-GM,其出瘤时间延长。结论:C57小鼠RMA-GM克隆细胞出瘤时间延长,肿瘤细胞免疫原性增强。
OBJECTIVE: To prepare a C57 mouse T lymphoma cell line that is highly expressed in GM-CSF. Methods: The mouse GM-CSF eukaryotic expression plasmid was electroporated into C57 murine T lymphoma cells. Single cell clone was prepared by double dilution method. The relative expression of GM-CSF was detected by RT-PCR, bone marrow stem cell proliferation assay and colony formation assay RMA-GM clone highly expressing GM-CSF. Results: The RMA-GM cell line with high expression of GM-CSF was obtained, and its tumor-bearing time was prolonged. Conclusion: The C57 mouse RMA-GM clone cells prolong the time of tumorigenesis and enhance the immunogenicity of tumor cells.